RT @crisprflydesign: That is not to say that there is no work in that direction. For example, several labs have shown that the activity of…
That is not to say that there is no work in that direction. For example, several labs have shown that the activity of different Cas12a enzymes are differentially affected by temperature. E.g.: https://t.co/qYusAippAY https://t.co/N8oUwRihd2 https://t.co
We also purified SpG/SpRY proteins obtaining similar results than using mRNA. Further, we “rescued” low efficient gRNAs that were likely unstable and got protected when forming RNP as we showed for other CRISPR-Cas systems. 7/11 https://t.co/TH8ooBYEKZ htt
@MStarostecka @Boutroslab @DKFZ Here we report a Cas12a system for Drosophila. Development has been crucially informed by previous work from @crisprscan @GiraldezLab showing that Cas12a enzymes are temperature sensitive. https://t.co/qYusAippAY
RT @doudna_lab: New Paper: CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. https://t.co/…
CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing published as: https://t.co/xCllUAR0lq @naturecomms #biorxiv
@scekker @DocDellaire @rolen_m @chrmosimann No idea. Dropping temperature worked quite well for editing in Zebrafish using Cas12a for HDR mediated repair https://t.co/Frf0M1ozwt
RT @doudna_lab: New Paper: CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. https://t.co/…
RT @crisprscan: https://t.co/TH8ooBYEKZ Our paper on Cpf1 is finally out! Check it out if you want to use this tool in vivo (including HDR…
Para cuándo una opción de archivar los tuits, @Twitter? https://t.co/mU3klyR1yR
@SoutoManel @Irreductible @Naukas_com La proteína #Cpf1 análoga a #Cas9 podría ser una solución alternativa. Se ha usado mucho para editar genéticamente plantas pero también es efectiva en animales, como ha demostrado hace poco M.A. Moreno-Mateos @crisprsc
RT @crisprscan: https://t.co/TH8ooBYEKZ Our paper on Cpf1 is finally out! Check it out if you want to use this tool in vivo (including HDR…
RT @doudna_lab: New Paper: CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. https://t.co/…
RT @doudna_lab: New Paper: CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. https://t.co/…
CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing - work from @GiraldezLab & @doudna_lab out in @NatureComms https://t.co/4HCg9YJtL1
RT @rosscloney: Temperature modulates AsCpf1 activity but less so for LbCpf1 https://t.co/1zeUULpqD0
RT @doudna_lab: New Paper: CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. https://t.co/…
RT @rosscloney: Temperature modulates AsCpf1 activity but less so for LbCpf1 https://t.co/1zeUULpqD0
Temperature modulates AsCpf1 activity but less so for LbCpf1 https://t.co/1zeUULpqD0
RT @doudna_lab: New Paper: CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. https://t.co/…
RT @DanveC: Temperature-regulated genome editing! @NatureComms https://t.co/gd1EgoQnxR
RT @DanveC: Temperature-regulated genome editing! @NatureComms https://t.co/gd1EgoQnxR
RT @doudna_lab: New Paper: CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. https://t.co/…
RT @doudna_lab: New Paper: CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. https://t.co/…
RT @doudna_lab: New Paper: CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. https://t.co/…
RT @doudna_lab: New Paper: CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. https://t.co/…
Thanks @doudna_lab and @crisprscan. It has been an honor working with you! https://t.co/w6IU52DxpR
RT @CRISPR_papers: CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. https://t.co/QXxCeS2y…
Temperature-regulated genome editing! @NatureComms https://t.co/gd1EgoQnxR
RT @doudna_lab: New Paper: CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. https://t.co/…
RT @doudna_lab: New Paper: CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. https://t.co/…
#CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing Nat Commun 1208 https://t.co/43UQZIQFcj https://t.co/pvu6iuBPvs
RT @doudna_lab: New Paper: CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. https://t.co/…
RT @doudna_lab: New Paper: CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. https://t.co/…
RT @doudna_lab: New Paper: CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. https://t.co/…
RT @doudna_lab: New Paper: CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. https://t.co/…
RT @doudna_lab: New Paper: CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. https://t.co/…
RT @doudna_lab: New Paper: CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. https://t.co/…
RT @doudna_lab: New Paper: CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. https://t.co/…
RT @doudna_lab: New Paper: CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. https://t.co/…
RT @doudna_lab: New Paper: CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. https://t.co/…
RT @doudna_lab: New Paper: CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. https://t.co/…
RT @doudna_lab: New Paper: CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. https://t.co/…
RT @doudna_lab: New Paper: CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. https://t.co/…
Some groups observed before the effect of temperature on Cpf1 activity. Good to know about the molecular mechanism! https://t.co/delk0zlEnS
RT @doudna_lab: New Paper: CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. https://t.co/…
RT @doudna_lab: New Paper: CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. https://t.co/…
RT @doudna_lab: New Paper: CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. https://t.co/…
RT @doudna_lab: New Paper: CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. https://t.co/…
RT @CRISPR_papers: CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. https://t.co/QXxCeS2y…
RT @doudna_lab: New Paper: CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. https://t.co/…
This woman is a rockstar https://t.co/ZcmMVX4CQF
RT @doudna_lab: New Paper: CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. https://t.co/…
CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. https://t.co/DHEpXWPYVU https://t.co/DX6VpVsELJ
New Paper: CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. https://t.co/DIkgoD6Hra
CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. https://t.co/V4emjx1vxu https://t.co/iKcxwHyANn
CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. https://t.co/QXxCeS2yox
RT @crisprscan: https://t.co/TH8ooBYEKZ Our paper on Cpf1 is finally out! Check it out if you want to use this tool in vivo (including HDR…
RT @crisprscan: https://t.co/TH8ooBYEKZ Our paper on Cpf1 is finally out! Check it out if you want to use this tool in vivo (including HDR…
RT @crisprscan: https://t.co/TH8ooBYEKZ Our paper on Cpf1 is finally out! Check it out if you want to use this tool in vivo (including HDR…
RT @crisprscan: https://t.co/TH8ooBYEKZ Our paper on Cpf1 is finally out! Check it out if you want to use this tool in vivo (including HDR…
RT @crisprscan: https://t.co/TH8ooBYEKZ Our paper on Cpf1 is finally out! Check it out if you want to use this tool in vivo (including HDR…
RT @crisprscan: https://t.co/TH8ooBYEKZ Our paper on Cpf1 is finally out! Check it out if you want to use this tool in vivo (including HDR…
RT @kmd6300: Use of LbCpf1 for increased HDR in zebrafish. #CRISPR https://t.co/u1CjxVqavU
RT @josefra1975: Congratulations for this new great achievement ;-) https://t.co/Ht3yKZ7fLO
Congratulations for this new great achievement ;-) https://t.co/Ht3yKZ7fLO
RT @crisprscan: https://t.co/TH8ooBYEKZ Our paper on Cpf1 is finally out! Check it out if you want to use this tool in vivo (including HDR…
https://t.co/TJX5UbhxU6 Congrats @crisprscan!
Use of LbCpf1 for increased HDR in zebrafish. #CRISPR https://t.co/u1CjxVqavU
It's a great story! Congrats! https://t.co/3ZIj3ePQrZ
https://t.co/TH8ooBYEKZ Our paper on Cpf1 is finally out! Check it out if you want to use this tool in vivo (including HDR approaches). Temperature is a key factor controlling Cpf1 activity.