| Title |
CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing
|
|---|---|
| Published in |
Nature Communications, December 2017
|
| DOI | 10.1038/s41467-017-01836-2 |
| Pubmed ID | |
| Authors |
Miguel A. Moreno-Mateos, Juan P. Fernandez, Romain Rouet, Charles E. Vejnar, Maura A. Lane, Emily Mis, Mustafa K. Khokha, Jennifer A. Doudna, Antonio J. Giraldez |
| Abstract |
Cpf1 is a novel class of CRISPR-Cas DNA endonucleases, with a wide range of activity across different eukaryotic systems. Yet, the underlying determinants of this variability are poorly understood. Here, we demonstrate that LbCpf1, but not AsCpf1, ribonucleoprotein complexes allow efficient mutagenesis in zebrafish and Xenopus. We show that temperature modulates Cpf1 activity by controlling its ability to access genomic DNA. This effect is stronger on AsCpf1, explaining its lower efficiency in ectothermic organisms. We capitalize on this property to show that temporal control of the temperature allows post-translational modulation of Cpf1-mediated genome editing. Finally, we determine that LbCpf1 significantly increases homology-directed repair in zebrafish, improving current approaches for targeted DNA integration in the genome. Together, we provide a molecular understanding of Cpf1 activity in vivo and establish Cpf1 as an efficient and inducible genome engineering tool across ectothermic species. |
X Demographics
The data shown below were collected from the profiles of 65 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 17 | 26% |
| Spain | 9 | 14% |
| United Kingdom | 6 | 9% |
| Australia | 3 | 5% |
| Canada | 3 | 5% |
| Netherlands | 2 | 3% |
| Germany | 2 | 3% |
| Norway | 1 | 2% |
| Russia | 1 | 2% |
| Other | 3 | 5% |
| Unknown | 18 | 28% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 33 | 51% |
| Scientists | 29 | 45% |
| Science communicators (journalists, bloggers, editors) | 3 | 5% |
Mendeley readers
The data shown below were compiled from readership statistics for 501 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 501 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 101 | 20% |
| Researcher | 91 | 18% |
| Student > Bachelor | 53 | 11% |
| Student > Master | 51 | 10% |
| Student > Doctoral Student | 26 | 5% |
| Other | 75 | 15% |
| Unknown | 104 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 202 | 40% |
| Agricultural and Biological Sciences | 116 | 23% |
| Neuroscience | 13 | 3% |
| Immunology and Microbiology | 11 | 2% |
| Medicine and Dentistry | 7 | 1% |
| Other | 38 | 8% |
| Unknown | 114 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 70. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 December 2022.
All research outputs
#595,803
of 25,088,711 outputs
Outputs from Nature Communications
#10,280
of 55,226 outputs
Outputs of similar age
#13,594
of 451,992 outputs
Outputs of similar age from Nature Communications
#304
of 1,439 outputs
Altmetric has tracked 25,088,711 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 97th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 55,226 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 55.9. This one has done well, scoring higher than 81% of its peers.
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We're also able to compare this research output to 1,439 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 78% of its contemporaries.