Title |
Telomere length regulates TERRA levels through increased trimethylation of telomeric H3K9 and HP1α
|
---|---|
Published in |
Nature Structural & Molecular Biology, August 2012
|
DOI | 10.1038/nsmb.2364 |
Pubmed ID | |
Authors |
Nausica Arnoult, Amandine Van Beneden, Anabelle Decottignies |
Abstract |
Gene silencing by the repressive telomeric chromatin environment, referred to as telomere position effect (TPE), has been well characterized in yeast and depends on telomere length. However, proof of its existence at native human chromosome ends has remained elusive, mainly owing to the paucity of genes near telomeres. The discovery of TERRAs, the telomeric noncoding RNAs transcribed from subtelomeric promoters, paved the way to probing for telomere-length impact on physiological TPE. Using cell lines of various origins, we show that telomere elongation consistently represses TERRA expression. Repression is mediated by increased trimethylated H3K9 density at telomeres and by heterochromatin protein HP1α, with no detectable spreading of the marks beyond the telomeric tract, restricting human TPE to telomere transcription. Our data further support the existence of a negative-feedback mechanism in which longer TERRA molecules repress their own transcription upon telomere elongation. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
France | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
France | 3 | 1% |
Japan | 3 | 1% |
United States | 2 | <1% |
Canada | 2 | <1% |
Brazil | 1 | <1% |
India | 1 | <1% |
Italy | 1 | <1% |
Netherlands | 1 | <1% |
Russia | 1 | <1% |
Other | 1 | <1% |
Unknown | 228 | 93% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 77 | 32% |
Researcher | 49 | 20% |
Student > Master | 25 | 10% |
Student > Bachelor | 16 | 7% |
Student > Doctoral Student | 11 | 5% |
Other | 30 | 12% |
Unknown | 36 | 15% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 96 | 39% |
Biochemistry, Genetics and Molecular Biology | 93 | 38% |
Chemistry | 4 | 2% |
Physics and Astronomy | 3 | 1% |
Medicine and Dentistry | 3 | 1% |
Other | 7 | 3% |
Unknown | 38 | 16% |