| Title |
Two-dimensional flow nanometry of biological nanoparticles for accurate determination of their size and emission intensity
|
|---|---|
| Published in |
Nature Communications, September 2016
|
| DOI | 10.1038/ncomms12956 |
| Pubmed ID | |
| Authors |
Stephan Block, Björn Johansson Fast, Anders Lundgren, Vladimir P. Zhdanov, Fredrik Höök |
| Abstract |
Biological nanoparticles (BNPs) are of high interest due to their key role in various biological processes and use as biomarkers. BNP size and composition are decisive for their functions, but simultaneous determination of both properties with high accuracy remains challenging. Optical microscopy allows precise determination of fluorescence/scattering intensity, but not the size of individual BNPs. The latter is better determined by tracking their random motion in bulk, but the limited illumination volume for tracking this motion impedes reliable intensity determination. Here, we show that by attaching BNPs to a supported lipid bilayer, subjecting them to hydrodynamic flows and tracking their motion via surface-sensitive optical imaging enable determination of their diffusion coefficients and flow-induced drifts, from which accurate quantification of both BNP size and emission intensity can be made. For vesicles, the accuracy of this approach is demonstrated by resolving the expected radius-squared dependence of their fluorescence intensity for radii down to 15 nm. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Sweden | 1 | 25% |
| Unknown | 3 | 75% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 75% |
| Scientists | 1 | 25% |
Mendeley readers
The data shown below were compiled from readership statistics for 38 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Sweden | 3 | 8% |
| Unknown | 35 | 92% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 15 | 39% |
| Researcher | 6 | 16% |
| Student > Master | 3 | 8% |
| Professor | 2 | 5% |
| Professor > Associate Professor | 2 | 5% |
| Other | 3 | 8% |
| Unknown | 7 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Physics and Astronomy | 10 | 26% |
| Biochemistry, Genetics and Molecular Biology | 5 | 13% |
| Chemistry | 5 | 13% |
| Engineering | 4 | 11% |
| Agricultural and Biological Sciences | 3 | 8% |
| Other | 2 | 5% |
| Unknown | 9 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 June 2017.
All research outputs
#5,774,273
of 23,881,329 outputs
Outputs from Nature Communications
#34,781
of 49,947 outputs
Outputs of similar age
#85,610
of 324,669 outputs
Outputs of similar age from Nature Communications
#591
of 861 outputs
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