| Title |
Stalking influenza by vaccination with pre-fusion headless HA mini-stem
|
|---|---|
| Published in |
Scientific Reports, March 2016
|
| DOI | 10.1038/srep22666 |
| Pubmed ID | |
| Authors |
Sophie A. Valkenburg, V. Vamsee Aditya Mallajosyula, Olive T. W. Li, Alex W. H. Chin, George Carnell, Nigel Temperton, Raghavan Varadarajan, Leo L. M. Poon |
| Abstract |
Inaccuracies in prediction of circulating viral strain genotypes and the possibility of novel reassortants causing a pandemic outbreak necessitate the development of an anti-influenza vaccine with increased breadth of protection and potential for rapid production and deployment. The hemagglutinin (HA) stem is a promising target for universal influenza vaccine as stem-specific antibodies have the potential to be broadly cross-reactive towards different HA subtypes. Here, we report the design of a bacterially expressed polypeptide that mimics a H5 HA stem by protein minimization to focus the antibody response towards the HA stem. The HA mini-stem folds as a trimer mimicking the HA prefusion conformation. It is resistant to thermal/chemical stress, and it binds to conformation-specific, HA stem-directed broadly neutralizing antibodies with high affinity. Mice vaccinated with the group 1 HA mini-stems are protected from morbidity and mortality against lethal challenge by both group 1 (H5 and H1) and group 2 (H3) influenza viruses, the first report of cross-group protection. Passive transfer of immune serum demonstrates the protection is mediated by stem-specific antibodies. Furthermore, antibodies indudced by these HA stems have broad HA reactivity, yet they do not have antibody-dependent enhancement activity. |
X Demographics
The data shown below were collected from the profiles of 12 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 3 | 25% |
| United States | 2 | 17% |
| Belgium | 1 | 8% |
| Brazil | 1 | 8% |
| Unknown | 5 | 42% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 10 | 83% |
| Scientists | 2 | 17% |
Mendeley readers
The data shown below were compiled from readership statistics for 126 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Netherlands | 1 | <1% |
| Unknown | 125 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 25 | 20% |
| Student > Bachelor | 20 | 16% |
| Researcher | 20 | 16% |
| Student > Master | 10 | 8% |
| Student > Doctoral Student | 4 | 3% |
| Other | 9 | 7% |
| Unknown | 38 | 30% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 21 | 17% |
| Immunology and Microbiology | 21 | 17% |
| Agricultural and Biological Sciences | 17 | 13% |
| Veterinary Science and Veterinary Medicine | 5 | 4% |
| Engineering | 5 | 4% |
| Other | 12 | 10% |
| Unknown | 45 | 36% |
Attention Score in Context
This research output has an Altmetric Attention Score of 18. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 February 2024.
All research outputs
#2,037,727
of 25,385,509 outputs
Outputs from Scientific Reports
#18,572
of 140,737 outputs
Outputs of similar age
#32,169
of 313,356 outputs
Outputs of similar age from Scientific Reports
#504
of 3,472 outputs
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