| Title |
A sequence-specific threading tetra-intercalator with an extremely slow dissociation rate constant
|
|---|---|
| Published in |
Nature Chemistry, September 2011
|
| DOI | 10.1038/nchem.1151 |
| Pubmed ID | |
| Authors |
Garen G. Holman, Maha Zewail-Foote, Amy Rhoden Smith, Kenneth A. Johnson, Brent L. Iverson |
| Abstract |
A long-lived and sequence-specific ligand-DNA complex would make possible the modulation of biological processes for extended periods. For this purpose, we are investigating a polyintercalation approach to DNA recognition in which flexible chains of aromatic units thread back and forth repeatedly through the double helix. Here we describe the DNA-binding behaviour of a threading tetra-intercalator. Specific binding was observed on a relatively long DNA strand that strongly favoured a predicted 14 base-pair sequence. Kinetic studies revealed a multistep association process, with sequence specificity that primarily derives from large differences in dissociation rates. The rate-limiting dissociation rate constant of the tetra-intercalator complex dissociating from its preferred binding site was extremely slow, corresponding to a half-life of 16 days. This is one of the longest non-covalent complex half-lives yet reported and, to the best of our knowledge, the longest for a DNA-binding molecule. |
Mendeley readers
The data shown below were compiled from readership statistics for 58 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 2% |
| Ecuador | 1 | 2% |
| Germany | 1 | 2% |
| Unknown | 55 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 16 | 28% |
| Researcher | 10 | 17% |
| Student > Master | 7 | 12% |
| Student > Bachelor | 6 | 10% |
| Student > Postgraduate | 4 | 7% |
| Other | 8 | 14% |
| Unknown | 7 | 12% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 39 | 67% |
| Agricultural and Biological Sciences | 5 | 9% |
| Unspecified | 2 | 3% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 3% |
| Neuroscience | 1 | 2% |
| Other | 1 | 2% |
| Unknown | 8 | 14% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 February 2012.
All research outputs
#21,447,530
of 23,942,155 outputs
Outputs from Nature Chemistry
#3,051
of 3,143 outputs
Outputs of similar age
#123,498
of 133,884 outputs
Outputs of similar age from Nature Chemistry
#49
of 49 outputs
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