↓ Skip to main content

A tissue-scale gradient of hydrogen peroxide mediates rapid wound detection in zebrafish

Overview of attention for article published in Nature, June 2009
Altmetric Badge

About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (97th percentile)
  • Above-average Attention Score compared to outputs of the same age and source (59th percentile)

Mentioned by

blogs
4 blogs
twitter
2 tweeters
patent
8 patents
wikipedia
1 Wikipedia page
f1000
1 research highlight platform

Citations

dimensions_citation
1079 Dimensions

Readers on

mendeley
885 Mendeley
citeulike
4 CiteULike
connotea
2 Connotea
Title
A tissue-scale gradient of hydrogen peroxide mediates rapid wound detection in zebrafish
Published in
Nature, June 2009
DOI 10.1038/nature08119
Pubmed ID
Authors

Philipp Niethammer, Clemens Grabher, A. Thomas Look, Timothy J. Mitchison

Abstract

Barrier structures (for example, epithelia around tissues and plasma membranes around cells) are required for internal homeostasis and protection from pathogens. Wound detection and healing represent a dormant morphogenetic program that can be rapidly executed to restore barrier integrity and tissue homeostasis. In animals, initial steps include recruitment of leukocytes to the site of injury across distances of hundreds of micrometres within minutes of wounding. The spatial signals that direct this immediate tissue response are unknown. Owing to their fast diffusion and versatile biological activities, reactive oxygen species, including hydrogen peroxide (H(2)O(2)), are interesting candidates for wound-to-leukocyte signalling. Here we probe the role of H(2)O(2) during the early events of wound responses in zebrafish larvae expressing a genetically encoded H(2)O(2) sensor. This reporter revealed a sustained rise in H(2)O(2) concentration at the wound margin, starting approximately 3 min after wounding and peaking at approximately 20 min, which extended approximately 100-200 microm into the tail-fin epithelium as a decreasing concentration gradient. Using pharmacological and genetic inhibition, we show that this gradient is created by dual oxidase (Duox), and that it is required for rapid recruitment of leukocytes to the wound. This is the first observation, to our knowledge, of a tissue-scale H(2)O(2) pattern, and the first evidence that H(2)O(2) signals to leukocytes in tissues, in addition to its known antiseptic role.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 885 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 18 2%
United Kingdom 12 1%
Portugal 4 <1%
Germany 4 <1%
Japan 4 <1%
France 4 <1%
Singapore 2 <1%
Chile 1 <1%
India 1 <1%
Other 7 <1%
Unknown 828 94%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 224 25%
Researcher 189 21%
Student > Master 91 10%
Student > Bachelor 84 9%
Professor > Associate Professor 59 7%
Other 168 19%
Unknown 70 8%
Readers by discipline Count As %
Agricultural and Biological Sciences 351 40%
Biochemistry, Genetics and Molecular Biology 164 19%
Medicine and Dentistry 64 7%
Chemistry 51 6%
Immunology and Microbiology 47 5%
Other 114 13%
Unknown 94 11%

Attention Score in Context

This research output has an Altmetric Attention Score of 39. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 May 2021.
All research outputs
#717,912
of 19,157,982 outputs
Outputs from Nature
#26,137
of 83,376 outputs
Outputs of similar age
#5,620
of 234,863 outputs
Outputs of similar age from Nature
#426
of 1,045 outputs
Altmetric has tracked 19,157,982 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 96th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 83,376 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 93.7. This one has gotten more attention than average, scoring higher than 68% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 234,863 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 97% of its contemporaries.
We're also able to compare this research output to 1,045 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 59% of its contemporaries.