| Title |
Ankyrin-mediated self-protection during cell invasion by the bacterial predator Bdellovibrio bacteriovorus
|
|---|---|
| Published in |
Nature Communications, December 2015
|
| DOI | 10.1038/ncomms9884 |
| Pubmed ID | |
| Authors |
Carey Lambert, Ian T. Cadby, Rob Till, Nhat Khai Bui, Thomas R. Lerner, William S. Hughes, David J. Lee, Luke J. Alderwick, Waldemar Vollmer, R. Elizabeth Sockett, Andrew L. Lovering |
| Abstract |
Predatory Bdellovibrio bacteriovorus are natural antimicrobial organisms, killing other bacteria by whole-cell invasion. Self-protection against prey-metabolizing enzymes is important for the evolution of predation. Initial prey entry involves the predator's peptidoglycan DD-endopeptidases, which decrosslink cell walls and prevent wasteful entry by a second predator. Here we identify and characterize a self-protection protein from B. bacteriovorus, Bd3460, which displays an ankyrin-based fold common to intracellular pathogens of eukaryotes. Co-crystal structures reveal Bd3460 complexation of dual targets, binding a conserved epitope of each of the Bd3459 and Bd0816 endopeptidases. Complexation inhibits endopeptidase activity and cell wall decrosslinking in vitro. Self-protection is vital - ΔBd3460 Bdellovibrio deleteriously decrosslink self-peptidoglycan upon invasion, adopt a round morphology, and lose predatory capacity and cellular integrity. Our analysis provides the first mechanistic examination of self-protection in Bdellovibrio, documents protection-multiplicity for products of two different genomic loci, and reveals an important evolutionary adaptation to an invasive predatory bacterial lifestyle. |
X Demographics
The data shown below were collected from the profiles of 36 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 9 | 25% |
| United States | 5 | 14% |
| France | 2 | 6% |
| India | 1 | 3% |
| Lithuania | 1 | 3% |
| Switzerland | 1 | 3% |
| Spain | 1 | 3% |
| Netherlands | 1 | 3% |
| Germany | 1 | 3% |
| Other | 0 | 0% |
| Unknown | 14 | 39% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 20 | 56% |
| Members of the public | 14 | 39% |
| Practitioners (doctors, other healthcare professionals) | 1 | 3% |
| Science communicators (journalists, bloggers, editors) | 1 | 3% |
Mendeley readers
The data shown below were compiled from readership statistics for 95 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 1% |
| Unknown | 94 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 21 | 22% |
| Student > Master | 18 | 19% |
| Student > Bachelor | 16 | 17% |
| Researcher | 7 | 7% |
| Student > Postgraduate | 4 | 4% |
| Other | 11 | 12% |
| Unknown | 18 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 26 | 27% |
| Agricultural and Biological Sciences | 25 | 26% |
| Immunology and Microbiology | 9 | 9% |
| Physics and Astronomy | 4 | 4% |
| Neuroscience | 2 | 2% |
| Other | 11 | 12% |
| Unknown | 18 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 132. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 October 2018.
All research outputs
#319,426
of 25,658,139 outputs
Outputs from Nature Communications
#4,865
of 57,993 outputs
Outputs of similar age
#4,999
of 396,918 outputs
Outputs of similar age from Nature Communications
#71
of 654 outputs
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