| Title |
Targeted sequencing reveals clonal genetic changes in the progression of early lung neoplasms and paired circulating DNA
|
|---|---|
| Published in |
Nature Communications, September 2015
|
| DOI | 10.1038/ncomms9258 |
| Pubmed ID | |
| Authors |
Evgeny Izumchenko, Xiaofei Chang, Mariana Brait, Elana Fertig, Luciane T. Kagohara, Atul Bedi, Luigi Marchionni, Nishant Agrawal, Rajani Ravi, Sian Jones, Mohammad O. Hoque, William H. Westra, David Sidransky |
| Abstract |
Lungs resected for adenocarcinomas often harbour minute discrete foci of cytologically atypical pneumocyte proliferations designated as atypical adenomatous hyperplasia (AAH). Evidence suggests that AAH represents an initial step in the progression to adenocarcinoma in situ (AIS), minimally invasive adenocarcinoma (MIA) and fully invasive adenocarcinoma. Despite efforts to identify predictive markers of malignant transformation, alterations driving this progression are poorly understood. Here we perform targeted next-generation sequencing on multifocal AAHs and different zones of histologic progression within AISs and MIAs. Multiregion sequencing demonstrated different genetic drivers within the same tumour and reveal that clonal expansion is an early event of tumorigenesis. We find that KRAS, TP53 and EGFR mutations are indicators of malignant transition. Utilizing droplet digital PCR, we find alterations associated with early neoplasms in paired circulating DNA. This study provides insight into the heterogeneity of clonal events in the progression of early lung neoplasia and demonstrates that these events can be detected even before neoplasms have invaded and acquired malignant potential. |
X Demographics
The data shown below were collected from the profiles of 17 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 5 | 29% |
| Spain | 2 | 12% |
| Puerto Rico | 1 | 6% |
| Ecuador | 1 | 6% |
| Unknown | 8 | 47% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 12 | 71% |
| Scientists | 5 | 29% |
Mendeley readers
The data shown below were compiled from readership statistics for 137 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| China | 1 | <1% |
| Ireland | 1 | <1% |
| France | 1 | <1% |
| Unknown | 134 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 30 | 22% |
| Student > Ph. D. Student | 27 | 20% |
| Student > Bachelor | 21 | 15% |
| Student > Master | 10 | 7% |
| Student > Doctoral Student | 8 | 6% |
| Other | 21 | 15% |
| Unknown | 20 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 39 | 28% |
| Agricultural and Biological Sciences | 31 | 23% |
| Biochemistry, Genetics and Molecular Biology | 30 | 22% |
| Nursing and Health Professions | 3 | 2% |
| Computer Science | 2 | 1% |
| Other | 8 | 6% |
| Unknown | 24 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 484. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 October 2017.
All research outputs
#52,889
of 24,938,276 outputs
Outputs from Nature Communications
#829
of 54,622 outputs
Outputs of similar age
#502
of 250,517 outputs
Outputs of similar age from Nature Communications
#9
of 772 outputs
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