Title |
Molecular recognition of wood polyphenols by phase II detoxification enzymes of the white rot Trametes versicolor
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Published in |
Scientific Reports, May 2018
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DOI | 10.1038/s41598-018-26601-3 |
Pubmed ID | |
Authors |
Mathieu Schwartz, Thomas Perrot, Emmanuel Aubert, Stéphane Dumarçay, Frédérique Favier, Philippe Gérardin, Mélanie Morel-Rouhier, Guillermo Mulliert, Fanny Saiag, Claude Didierjean, Eric Gelhaye |
Abstract |
Wood decay fungi have complex detoxification systems that enable them to cope with secondary metabolites produced by plants. Although the number of genes encoding for glutathione transferases is especially expanded in lignolytic fungi, little is known about their target molecules. In this study, by combining biochemical, enzymatic and structural approaches, interactions between polyphenols and six glutathione transferases from the white-rot fungus Trametes versicolor have been demonstrated. Two isoforms, named TvGSTO3S and TvGSTO6S have been deeply studied at the structural level. Each isoform shows two distinct ligand-binding sites, a narrow L-site at the dimer interface and a peculiar deep hydrophobic H-site. In TvGSTO3S, the latter appears optimized for aromatic ligand binding such as hydroxybenzophenones. Affinity crystallography revealed that this H-site retains the flavonoid dihydrowogonin from a partially purified wild-cherry extract. Besides, TvGSTO6S binds two molecules of the flavonoid naringenin in the L-site. These data suggest that TvGSTO isoforms could interact with plant polyphenols released during wood degradation. |
X Demographics
Geographical breakdown
Country | Count | As % |
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France | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 50% |
Science communicators (journalists, bloggers, editors) | 1 | 50% |
Mendeley readers
Geographical breakdown
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Unknown | 32 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 7 | 22% |
Researcher | 4 | 13% |
Student > Master | 3 | 9% |
Student > Doctoral Student | 2 | 6% |
Student > Bachelor | 2 | 6% |
Other | 4 | 13% |
Unknown | 10 | 31% |
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Unspecified | 1 | 3% |
Environmental Science | 1 | 3% |
Earth and Planetary Sciences | 1 | 3% |
Other | 2 | 6% |
Unknown | 15 | 47% |