| Title |
Elevation of the provitamin A content of transgenic tomato plants
|
|---|---|
| Published in |
Nature Biotechnology, June 2000
|
| DOI | 10.1038/76523 |
| Pubmed ID | |
| Authors |
Susanne Römer, Paul D. Fraser, Joy W. Kiano, Cathie A. Shipton, Norihiko Misawa, Wolfgang Schuch, Peter M. Bramley |
| Abstract |
Tomato products are the principal dietary sources of lycopene and major source of beta-carotene, both of which have been shown to benefit human health. To enhance the carotenoid content and profile of tomato fruit, we have produced transgenic lines containing a bacterial carotenoid gene (crtI) encoding the enzyme phytoene desaturase, which converts phytoene into lycopene. Expression of this gene in transgenic tomatoes did not elevate total carotenoid levels. However, the beta-carotene content increased about threefold, up to 45% of the total carotenoid content. Endogenous carotenoid genes were concurrently upregulated, except for phytoene synthase, which was repressed. The alteration in carotenoid content of these plants did not affect growth and development. Levels of noncarotenoid isoprenoids were unchanged in the transformants. The phenotype has been found to be stable and reproducible over at least four generations. |
Mendeley readers
The data shown below were compiled from readership statistics for 165 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 2 | 1% |
| Spain | 2 | 1% |
| Hungary | 1 | <1% |
| Netherlands | 1 | <1% |
| United Kingdom | 1 | <1% |
| Italy | 1 | <1% |
| Greece | 1 | <1% |
| United States | 1 | <1% |
| Unknown | 155 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 35 | 21% |
| Student > Ph. D. Student | 32 | 19% |
| Student > Bachelor | 19 | 12% |
| Professor | 16 | 10% |
| Student > Master | 16 | 10% |
| Other | 23 | 14% |
| Unknown | 24 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 88 | 53% |
| Biochemistry, Genetics and Molecular Biology | 25 | 15% |
| Chemistry | 7 | 4% |
| Medicine and Dentistry | 4 | 2% |
| Computer Science | 3 | 2% |
| Other | 11 | 7% |
| Unknown | 27 | 16% |
Attention Score in Context
This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 May 2022.
All research outputs
#3,271,353
of 22,782,096 outputs
Outputs from Nature Biotechnology
#3,646
of 8,201 outputs
Outputs of similar age
#3,182
of 39,160 outputs
Outputs of similar age from Nature Biotechnology
#9
of 51 outputs
Altmetric has tracked 22,782,096 research outputs across all sources so far. Compared to these this one has done well and is in the 84th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 8,201 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 40.1. This one has gotten more attention than average, scoring higher than 50% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 39,160 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 87% of its contemporaries.
We're also able to compare this research output to 51 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 68% of its contemporaries.