2/7 In the original mux-seq workflow, we utilized genetic variants as natural barcodes to enable highly systematic and cost effective single-cell sequencing. But to demultiplex, we needed reference genotypes available which may not always be possible. http
The mux-seq approach @NatureBiotech https://t.co/LjXpfCcBqS #HCAAsia2022
@_nicoromano_ Demuxlet, because genotypes don’t lie for pooled experiments. https://t.co/GhWw7emu4l
@JunhyongKim @jmurray Apologies to the authors of the earlier work which we should have cited. We'll correct that in the newer version https://t.co/TsrXobrnWJ
For their protocol *s > 100 and they give an approximate formula, simulation, and empirical evidence that we can push close to 100% usably loaded droplets. A different idea is this older paper https://t.co/VYddhzFPKS called demuxlet (which the above pa
RT @patrlundgren: Interesting. Looking forward to see how single cell genomics becomes integrated with drug development in the next 5-10 ye…
Interesting. Looking forward to see how single cell genomics becomes integrated with drug development in the next 5-10 years. Super-saturation loading and demultiplexing based on human genotypes (https://t.co/9EczqEF3r3) will likely be useful to increase s
RT @cmcginnisUCSF: @LGMartelotto (18/20) In silico genotyping Demuxlet (@yimmieg): https://t.co/OBZFMjqOmk Vireo (@davisjmcc): https://t.c…
RT @cmcginnisUCSF: @LGMartelotto (18/20) In silico genotyping Demuxlet (@yimmieg): https://t.co/OBZFMjqOmk Vireo (@davisjmcc): https://t.c…
@LGMartelotto (18/20) In silico genotyping Demuxlet (@yimmieg): https://t.co/OBZFMjqOmk Vireo (@davisjmcc): https://t.co/1P5U2Klqhe Souporcell (@m_hemberg + @marakat): https://t.co/zy7ziqdYr9 (Let me know of others I’ve missed!)
JY: Overload the droplets in dropseq/10X to increase the throughput. Multiplex cells from different individuals together. As long as they are genetically different. Originally this required prior genetic information: https://t.co/PSrt2E36yW #singlecell
RT @YiXing77: Hyun Min Kang of Michigan talking about population scale single cell RNAseq using genetic multiplexing https://t.co/EshSRpt6e…
Hyun Min Kang of Michigan talking about population scale single cell RNAseq using genetic multiplexing https://t.co/EshSRpt6eb @UCLA_CGSI #cgsi2019 https://t.co/ahTRfphXr3
- CellTag Indexing (https://t.co/3VtqTw2QPa) @morris_lab - Demuxlet (https://t.co/ihQ7QGXLXg) @yimmieg - Click Tags (https://t.co/c4XpCFoJp7) @sisichen @lpachter - Transient barcoding (https://t.co/nRElGnXWyt)
Julie Jerber — GWAS and eQTLs. Overcome limitations using iHPS. However, one cell line at a time => sample collection and pooling and scRNA-seq (demuxlet: https://t.co/q31pVVFazR) #BoG19
@cleidson_alves you can use https://t.co/n87aa0T8Fi
Multiplexed droplet single-cell RNA-sequencing using natural genetic variation. https://t.co/Nj1tZXTJBy #Neuralstemcells
RT @NYGCtech: alternative / complementary approaches: https://t.co/oQjOBjlvnb (@yimmieg) https://t.co/1ODZr672nb (@lpachter) https://t.co/7…
RT @NYGCtech: alternative / complementary approaches: https://t.co/oQjOBjlvnb (@yimmieg) https://t.co/1ODZr672nb (@lpachter) https://t.co/7…
RT @NYGCtech: alternative / complementary approaches: https://t.co/oQjOBjlvnb (@yimmieg) https://t.co/1ODZr672nb (@lpachter) https://t.co/7…
alternative / complementary approaches: https://t.co/oQjOBjlvnb (@yimmieg) https://t.co/1ODZr672nb (@lpachter) https://t.co/72RsGi10tr (Gartner lab: @cmcginnisUCSF) https://t.co/i20Gw40Xjw (Bang lab) https://t.co/WU7naWqzbM (@morris_lab) https://t.co/oZtAF
RT @coregenomics: Just saw that @yimmieg @NatureBiotech paper was featured as Stephen Sawcer's choice in his @CEGX_news interview...https:/…
RT @yimmieg: Awesome! https://t.co/wO4pfy611y
RT @coregenomics: Just saw that @yimmieg @NatureBiotech paper was featured as Stephen Sawcer's choice in his @CEGX_news interview...https:/…
Awesome!
Just saw that @yimmieg @NatureBiotech paper was featured as Stephen Sawcer's choice in his @CEGX_news interview...https://t.co/9hr4O9zo9g Read it at: https://t.co/7tAKTJXEVz https://t.co/O2QAbExC2c
Meena Subramaniam: read more at https://t.co/dIwvJ1JLqH #BoG18
RT @yimmieg: Officially published! First lab paper describing a method to decrease cost and improve experimental design for population scal…
Multiplexed droplet single-cell RNA-sequencing using natural genetic variation https://t.co/fSIHBW0sXy
RT @TorontoGenomics: SZ: talking about this great paper from Kang et al. https://t.co/brJKdTnXl7 - the demuxlet method to use naturally occ…
SZ: talking about this great paper from Kang et al. https://t.co/brJKdTnXl7 - the demuxlet method to use naturally occuring SNPs to parse out sample of origin in muliplexed scRNA-seq #scgd18
RT @yimmieg: Officially published! First lab paper describing a method to decrease cost and improve experimental design for population scal…
RT @10xgenomics: New pub in @NatureBiotech - Multiplexed droplet single-cell RNA-sequencing using natural genetic variation - introduces a…
Multiplexed dscRNA-seq of pooled cells from unrelated individuals and double detection enabled by Demuxlet using @10xgenomics #SingleCell #RNAseq #Multiplex #transcriptomics https://t.co/1I2AMwWK9H
RT @10xgenomics: New pub in @NatureBiotech - Multiplexed droplet single-cell RNA-sequencing using natural genetic variation - introduces a…
RT @10xgenomics: New pub in @NatureBiotech - Multiplexed droplet single-cell RNA-sequencing using natural genetic variation - introduces a…
RT @10xgenomics: New pub in @NatureBiotech - Multiplexed droplet single-cell RNA-sequencing using natural genetic variation - introduces a…
RT @10xgenomics: New pub in @NatureBiotech - Multiplexed droplet single-cell RNA-sequencing using natural genetic variation - introduces a…
New pub in @NatureBiotech - Multiplexed droplet single-cell RNA-sequencing using natural genetic variation - introduces a new computational tool, demuxlet, for doublet detection & multiplexed #singlecell #RNAseq of pooled cells from unrelated individua
RT @10xgenomics: New pub in @NatureBiotech - Multiplexed droplet single-cell RNA-sequencing using natural genetic variation - introduces a…
RT @10xgenomics: New pub in @NatureBiotech - Multiplexed droplet single-cell RNA-sequencing using natural genetic variation - introduces a…
RT @10xgenomics: New pub in @NatureBiotech - Multiplexed droplet single-cell RNA-sequencing using natural genetic variation - introduces a…
RT @10xgenomics: New pub in @NatureBiotech - Multiplexed droplet single-cell RNA-sequencing using natural genetic variation - introduces a…
RT @10xgenomics: New pub in @NatureBiotech - Multiplexed droplet single-cell RNA-sequencing using natural genetic variation - introduces a…
New pub in @NatureBiotech - Multiplexed droplet single-cell RNA-sequencing using natural genetic variation - introduces a new computational tool, demuxlet, for doublet detection & multiplexed #singlecell #RNAseq of pooled cells from unrelated individua
RT @NatureBiotech: Multiplexed droplet single-cell RNA-sequencing using natural genetic variation https://t.co/ftJQEuuWFk #genomeinformatic…
RT @10xgenomics: New pub in @NatureBiotech - Multiplexed droplet single-cell RNA-sequencing using natural genetic variation - introduces a…
@satijalab @10xgenomics @m_stoeckius @swzheng29 @psmibert @nygenome inspired by, and complementary to demuxlet from @yimmieg https://t.co/oQjOBjlvnb
RT @10xgenomics: New pub in @NatureBiotech - Multiplexed droplet single-cell RNA-sequencing using natural genetic variation - introduces a…
RT @10xgenomics: New pub in @NatureBiotech - Multiplexed droplet single-cell RNA-sequencing using natural genetic variation - introduces a…
RT @10xgenomics: New pub in @NatureBiotech - Multiplexed droplet single-cell RNA-sequencing using natural genetic variation - introduces a…
RT @10xgenomics: New pub in @NatureBiotech - Multiplexed droplet single-cell RNA-sequencing using natural genetic variation - introduces a…
RT @10xgenomics: New pub in @NatureBiotech - Multiplexed droplet single-cell RNA-sequencing using natural genetic variation - introduces a…
RT @10xgenomics: New pub in @NatureBiotech - Multiplexed droplet single-cell RNA-sequencing using natural genetic variation - introduces a…
RT @10xgenomics: New pub in @NatureBiotech - Multiplexed droplet single-cell RNA-sequencing using natural genetic variation - introduces a…
RT @10xgenomics: New pub in @NatureBiotech - Multiplexed droplet single-cell RNA-sequencing using natural genetic variation - introduces a…
RT @10xgenomics: New pub in @NatureBiotech - Multiplexed droplet single-cell RNA-sequencing using natural genetic variation - introduces a…
New pub in @NatureBiotech - Multiplexed droplet single-cell RNA-sequencing using natural genetic variation - introduces a new computational tool, demuxlet, for doublet detection & multiplexed #singlecell #RNAseq of pooled cells from unrelated individua
RT @yimmieg: Officially published! First lab paper describing a method to decrease cost and improve experimental design for population scal…
RT @yimmieg: Officially published! First lab paper describing a method to decrease cost and improve experimental design for population scal…
RT @yimmieg: Officially published! First lab paper describing a method to decrease cost and improve experimental design for population scal…
RT @atulbutte: Great paper from @UCSF_ICHS people, including @yimmieg, shows how to get to population scale single-cell sequencing! https:/…
RT @atulbutte: Great paper from @UCSF_ICHS people, including @yimmieg, shows how to get to population scale single-cell sequencing! https:/…
RT @atulbutte: Great paper from @UCSF_ICHS people, including @yimmieg, shows how to get to population scale single-cell sequencing! https:/…
RT @atulbutte: Great paper from @UCSF_ICHS people, including @yimmieg, shows how to get to population scale single-cell sequencing! https:/…
RT @yimmieg: Officially published! First lab paper describing a method to decrease cost and improve experimental design for population scal…
RT @atulbutte: Great paper from @UCSF_ICHS people, including @yimmieg, shows how to get to population scale single-cell sequencing! https:/…
RT @yimmieg: Officially published! First lab paper describing a method to decrease cost and improve experimental design for population scal…
RT @atulbutte: Great paper from @UCSF_ICHS people, including @yimmieg, shows how to get to population scale single-cell sequencing! https:/…
RT @NatureBiotech: Multiplexed droplet single-cell RNA-sequencing using natural genetic variation https://t.co/ftJQEuuWFk #genomeinformatic…
RT @atulbutte: Great paper from @UCSF_ICHS people, including @yimmieg, shows how to get to population scale single-cell sequencing! https:/…
RT @atulbutte: Great paper from @UCSF_ICHS people, including @yimmieg, shows how to get to population scale single-cell sequencing! https:/…
RT @atulbutte: Great paper from @UCSF_ICHS people, including @yimmieg, shows how to get to population scale single-cell sequencing! https:/…
RT @atulbutte: Great paper from @UCSF_ICHS people, including @yimmieg, shows how to get to population scale single-cell sequencing! https:/…
RT @atulbutte: Great paper from @UCSF_ICHS people, including @yimmieg, shows how to get to population scale single-cell sequencing! https:/…
RT @atulbutte: Great paper from @UCSF_ICHS people, including @yimmieg, shows how to get to population scale single-cell sequencing! https:/…
RT @atulbutte: Great paper from @UCSF_ICHS people, including @yimmieg, shows how to get to population scale single-cell sequencing! https:/…
RT @atulbutte: Great paper from @UCSF_ICHS people, including @yimmieg, shows how to get to population scale single-cell sequencing! https:/…
RT @atulbutte: Great paper from @UCSF_ICHS people, including @yimmieg, shows how to get to population scale single-cell sequencing! https:/…
RT @atulbutte: Great paper from @UCSF_ICHS people, including @yimmieg, shows how to get to population scale single-cell sequencing! https:/…
RT @atulbutte: Great paper from @UCSF_ICHS people, including @yimmieg, shows how to get to population scale single-cell sequencing! https:/…
RT @atulbutte: Great paper from @UCSF_ICHS people, including @yimmieg, shows how to get to population scale single-cell sequencing! https:/…
RT @atulbutte: Great paper from @UCSF_ICHS people, including @yimmieg, shows how to get to population scale single-cell sequencing! https:/…
RT @yimmieg: Officially published! First lab paper describing a method to decrease cost and improve experimental design for population scal…
RT @NatureBiotech: Multiplexed droplet single-cell RNA-sequencing using natural genetic variation https://t.co/ftJQEuuWFk #genomeinformatic…
RT @yimmieg: Officially published! First lab paper describing a method to decrease cost and improve experimental design for population scal…
RT @yimmieg: Officially published! First lab paper describing a method to decrease cost and improve experimental design for population scal…
RT @yimmieg: Officially published! First lab paper describing a method to decrease cost and improve experimental design for population scal…
RT @yimmieg: Officially published! First lab paper describing a method to decrease cost and improve experimental design for population scal…
RT @yimmieg: Officially published! First lab paper describing a method to decrease cost and improve experimental design for population scal…
RT @yimmieg: Officially published! First lab paper describing a method to decrease cost and improve experimental design for population scal…
RT @yimmieg: Officially published! First lab paper describing a method to decrease cost and improve experimental design for population scal…
RT @NatureBiotech: Multiplexed droplet single-cell RNA-sequencing using natural genetic variation https://t.co/ftJQEuuWFk #genomeinformatic…
RT @scell_papers: Multiplexed droplet single-cell RNA-sequencing using natural genetic variation. https://t.co/YWZrZp0LWj #NatBiotechnol