| Title |
Universal sample preparation method for proteome analysis
|
|---|---|
| Published in |
Nature Methods, April 2009
|
| DOI | 10.1038/nmeth.1322 |
| Pubmed ID | |
| Authors |
Jacek R Wiśniewski, Alexandre Zougman, Nagarjuna Nagaraj, Matthias Mann |
| Abstract |
We describe a method, filter-aided sample preparation (FASP), which combines the advantages of in-gel and in-solution digestion for mass spectrometry-based proteomics. We completely solubilized the proteome in sodium dodecyl sulfate, which we then exchanged by urea on a standard filtration device. Peptides eluted after digestion on the filter were pure, allowing single-run analyses of organelles and an unprecedented depth of proteome coverage. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 3,418 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 35 | 1% |
| Germany | 16 | <1% |
| United Kingdom | 14 | <1% |
| Spain | 8 | <1% |
| Netherlands | 6 | <1% |
| South Africa | 5 | <1% |
| India | 5 | <1% |
| Brazil | 4 | <1% |
| Italy | 3 | <1% |
| Other | 29 | <1% |
| Unknown | 3293 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 866 | 25% |
| Researcher | 691 | 20% |
| Student > Master | 396 | 12% |
| Student > Bachelor | 277 | 8% |
| Student > Doctoral Student | 182 | 5% |
| Other | 448 | 13% |
| Unknown | 558 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 1031 | 30% |
| Biochemistry, Genetics and Molecular Biology | 817 | 24% |
| Chemistry | 280 | 8% |
| Medicine and Dentistry | 146 | 4% |
| Immunology and Microbiology | 98 | 3% |
| Other | 362 | 11% |
| Unknown | 684 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 54. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 March 2024.
All research outputs
#803,289
of 26,017,215 outputs
Outputs from Nature Methods
#1,048
of 5,401 outputs
Outputs of similar age
#1,853
of 111,000 outputs
Outputs of similar age from Nature Methods
#3
of 46 outputs
Altmetric has tracked 26,017,215 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 96th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 5,401 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 36.7. This one has done well, scoring higher than 80% of its peers.
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We're also able to compare this research output to 46 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 93% of its contemporaries.