| Title |
Inducible control of gene expression with destabilized Cre
|
|---|---|
| Published in |
Nature Methods, September 2013
|
| DOI | 10.1038/nmeth.2640 |
| Pubmed ID | |
| Authors |
Richard Sando, Karsten Baumgaertel, Simon Pieraut, Nina Torabi-Rander, Thomas J Wandless, Mark Mayford, Anton Maximov |
| Abstract |
Acute manipulation of gene and protein function in the brain is essential for understanding the mechanisms of nervous system development, plasticity and information processing. Here we describe a technique based on a destabilized Cre recombinase (DD-Cre) whose activity is controlled by the antibiotic trimethoprim (TMP). We show that DD-Cre triggers rapid TMP-dependent recombination of loxP-flanked ('floxed') alleles in mouse neurons in vivo and validate the use of this system for neurobehavioral research. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 2 | 50% |
| United States | 1 | 25% |
| Unknown | 1 | 25% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 2 | 50% |
| Members of the public | 2 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 322 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 8 | 2% |
| Switzerland | 2 | <1% |
| China | 2 | <1% |
| France | 1 | <1% |
| Austria | 1 | <1% |
| Germany | 1 | <1% |
| United Kingdom | 1 | <1% |
| Japan | 1 | <1% |
| Korea, Republic of | 1 | <1% |
| Other | 0 | 0% |
| Unknown | 304 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 94 | 29% |
| Student > Ph. D. Student | 77 | 24% |
| Student > Bachelor | 24 | 7% |
| Professor | 19 | 6% |
| Student > Master | 18 | 6% |
| Other | 56 | 17% |
| Unknown | 34 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 140 | 43% |
| Neuroscience | 71 | 22% |
| Biochemistry, Genetics and Molecular Biology | 38 | 12% |
| Medicine and Dentistry | 10 | 3% |
| Chemistry | 10 | 3% |
| Other | 17 | 5% |
| Unknown | 36 | 11% |
Attention Score in Context
This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 September 2022.
All research outputs
#6,221,646
of 23,371,053 outputs
Outputs from Nature Methods
#3,414
of 5,008 outputs
Outputs of similar age
#53,662
of 203,805 outputs
Outputs of similar age from Nature Methods
#61
of 91 outputs
Altmetric has tracked 23,371,053 research outputs across all sources so far. This one has received more attention than most of these and is in the 73rd percentile.
So far Altmetric has tracked 5,008 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 35.2. This one is in the 31st percentile – i.e., 31% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 203,805 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.
We're also able to compare this research output to 91 others from the same source and published within six weeks on either side of this one. This one is in the 32nd percentile – i.e., 32% of its contemporaries scored the same or lower than it.