| Title |
RNAi screens for Rho GTPase regulators of cell shape and YAP/TAZ localisation in triple negative breast cancer
|
|---|---|
| Published in |
Scientific Data, March 2017
|
| DOI | 10.1038/sdata.2017.18 |
| Pubmed ID | |
| Authors |
Patricia Pascual-Vargas, Samuel Cooper, Julia Sero, Vicky Bousgouni, Mar Arias-Garcia, Chris Bakal |
| Abstract |
In order to metastasise, triple negative breast cancer (TNBC) must make dynamic changes in cell shape. The shape of all eukaryotic cells is regulated by Rho Guanine Nucleotide Exchange Factors (RhoGEFs), which activate Rho-family GTPases in response to mechanical and informational cues. In contrast, Rho GTPase-activating proteins (RhoGAPs) inhibit Rho GTPases. However, which RhoGEFs and RhoGAPS couple TNBC cell shape to changes in their environment is very poorly understood. Moreover, whether the activity of particular RhoGEFs and RhoGAPs become dysregulated as cells evolve the ability to metastasise is not clear. Towards the ultimate goal of identifying RhoGEFs and RhoGAPs that are essential for TNBC metastasis, we performed an RNAi screen to isolate RhoGEFs and RhoGAPs that contribute to the morphogenesis of the highly metastatic TNBC cell line LM2, and its less-metastatic parental cell line MDA-MB-231. For ~6 million cells from each cell line, we measured 127 different features following the depletion of 142 genes. Using a linear classifier scheme we also describe the morphological heterogeneity of each gene-depleted population. |
X Demographics
The data shown below were collected from the profiles of 30 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 12 | 40% |
| United States | 2 | 7% |
| Germany | 2 | 7% |
| Australia | 2 | 7% |
| Turkey | 1 | 3% |
| Netherlands | 1 | 3% |
| Austria | 1 | 3% |
| Ecuador | 1 | 3% |
| Israel | 1 | 3% |
| Other | 2 | 7% |
| Unknown | 5 | 17% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 16 | 53% |
| Scientists | 12 | 40% |
| Science communicators (journalists, bloggers, editors) | 1 | 3% |
| Practitioners (doctors, other healthcare professionals) | 1 | 3% |
Mendeley readers
The data shown below were compiled from readership statistics for 73 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 73 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 17 | 23% |
| Student > Ph. D. Student | 13 | 18% |
| Student > Bachelor | 9 | 12% |
| Student > Doctoral Student | 4 | 5% |
| Professor > Associate Professor | 4 | 5% |
| Other | 9 | 12% |
| Unknown | 17 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 27 | 37% |
| Agricultural and Biological Sciences | 12 | 16% |
| Engineering | 6 | 8% |
| Immunology and Microbiology | 2 | 3% |
| Nursing and Health Professions | 1 | 1% |
| Other | 5 | 7% |
| Unknown | 20 | 27% |
Attention Score in Context
This research output has an Altmetric Attention Score of 18. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 May 2020.
All research outputs
#2,048,117
of 25,200,621 outputs
Outputs from Scientific Data
#828
of 3,221 outputs
Outputs of similar age
#38,360
of 317,295 outputs
Outputs of similar age from Scientific Data
#22
of 59 outputs
Altmetric has tracked 25,200,621 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 91st percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,221 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 22.4. This one has gotten more attention than average, scoring higher than 74% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 317,295 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 87% of its contemporaries.
We're also able to compare this research output to 59 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 64% of its contemporaries.