| Title |
A new type V toxin-antitoxin system where mRNA for toxin GhoT is cleaved by antitoxin GhoS
|
|---|---|
| Published in |
Nature Chemical Biology, September 2012
|
| DOI | 10.1038/nchembio.1062 |
| Pubmed ID | |
| Authors |
Xiaoxue Wang, Dana M Lord, Hsin-Yao Cheng, Devon O Osbourne, Seok Hoon Hong, Viviana Sanchez-Torres, Cecilia Quiroga, Kevin Zheng, Torsten Herrmann, Wolfgang Peti, Michael J Benedik, Rebecca Page, Thomas K Wood |
| Abstract |
Among bacterial toxin-antitoxin systems, to date no antitoxin has been identified that functions by cleaving toxin mRNA. Here we show that YjdO (renamed GhoT) is a membrane lytic peptide that causes ghost cell formation (lysed cells with damaged membranes) and increases persistence (persister cells are tolerant to antibiotics without undergoing genetic change). GhoT is part of a new toxin-antitoxin system with YjdK (renamed GhoS) because in vitro RNA degradation studies, quantitative real-time reverse-transcription PCR and whole-transcriptome studies revealed that GhoS masks GhoT toxicity by cleaving specifically yjdO (ghoT) mRNA. Alanine substitutions showed that Arg28 is important for GhoS activity, and RNA sequencing indicated that the GhoS cleavage site is rich in U and A. The NMR structure of GhoS indicates it is related to the CRISPR-associated-2 RNase, and GhoS is a monomer. Hence, GhoT-GhoS is to our knowledge the first type V toxin-antitoxin system where a protein antitoxin inhibits the toxin by cleaving specifically its mRNA. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 50% |
| United States | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 314 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 2 | <1% |
| Belgium | 2 | <1% |
| United States | 2 | <1% |
| Korea, Republic of | 1 | <1% |
| India | 1 | <1% |
| Indonesia | 1 | <1% |
| Slovakia | 1 | <1% |
| Germany | 1 | <1% |
| Estonia | 1 | <1% |
| Other | 1 | <1% |
| Unknown | 301 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 88 | 28% |
| Student > Master | 52 | 17% |
| Researcher | 48 | 15% |
| Student > Bachelor | 35 | 11% |
| Professor > Associate Professor | 9 | 3% |
| Other | 30 | 10% |
| Unknown | 52 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 114 | 36% |
| Biochemistry, Genetics and Molecular Biology | 94 | 30% |
| Immunology and Microbiology | 16 | 5% |
| Engineering | 10 | 3% |
| Chemical Engineering | 5 | 2% |
| Other | 18 | 6% |
| Unknown | 57 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 19. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 December 2020.
All research outputs
#1,666,690
of 22,675,759 outputs
Outputs from Nature Chemical Biology
#996
of 3,054 outputs
Outputs of similar age
#11,029
of 170,475 outputs
Outputs of similar age from Nature Chemical Biology
#6
of 38 outputs
Altmetric has tracked 22,675,759 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 92nd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,054 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 25.3. This one has gotten more attention than average, scoring higher than 67% of its peers.
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We're also able to compare this research output to 38 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 84% of its contemporaries.