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DNA-guided genome editing using the Natronobacterium gregoryi Argonaute

Overview of attention for article published in Nature Biotechnology, May 2016
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (99th percentile)
  • High Attention Score compared to outputs of the same age and source (98th percentile)

Citations

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136 Dimensions

Readers on

mendeley
1584 Mendeley
citeulike
8 CiteULike
Title
DNA-guided genome editing using the Natronobacterium gregoryi Argonaute
Published in
Nature Biotechnology, May 2016
DOI 10.1038/nbt.3547
Pubmed ID
Authors

Feng Gao, Xiao Z Shen, Feng Jiang, Yongqiang Wu, Chunyu Han

Abstract

The RNA-guided endonuclease Cas9 has made genome editing a widely accessible technique. Similar to Cas9, endonucleases from the Argonaute protein family also use oligonucleotides as guides to degrade invasive genomes. Here we report that the Natronobacterium gregoryi Argonaute (NgAgo) is a DNA-guided endonuclease suitable for genome editing in human cells. NgAgo binds 5' phosphorylated single-stranded guide DNA (gDNA) of ∼24 nucleotides, efficiently creates site-specific DNA double-strand breaks when loaded with the gDNA. The NgAgo-gDNA system does not require a protospacer-adjacent motif (PAM), as does Cas9, and preliminary characterization suggests a low tolerance to guide-target mismatches and high efficiency in editing (G+C)-rich genomic targets.

Twitter Demographics

The data shown below were collected from the profiles of 367 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 1,584 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 28 2%
Germany 8 <1%
China 8 <1%
United Kingdom 6 <1%
Denmark 4 <1%
Austria 4 <1%
Belgium 3 <1%
Japan 3 <1%
Mexico 3 <1%
Other 28 2%
Unknown 1489 94%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 458 29%
Researcher 363 23%
Student > Master 190 12%
Student > Bachelor 134 8%
Student > Doctoral Student 78 5%
Other 245 15%
Unknown 116 7%
Readers by discipline Count As %
Agricultural and Biological Sciences 634 40%
Biochemistry, Genetics and Molecular Biology 531 34%
Medicine and Dentistry 56 4%
Neuroscience 35 2%
Chemistry 35 2%
Other 140 9%
Unknown 153 10%

Attention Score in Context

This research output has an Altmetric Attention Score of 476. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 September 2021.
All research outputs
#33,951
of 19,157,212 outputs
Outputs from Nature Biotechnology
#71
of 7,622 outputs
Outputs of similar age
#924
of 273,397 outputs
Outputs of similar age from Nature Biotechnology
#3
of 119 outputs
Altmetric has tracked 19,157,212 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 7,622 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 36.4. This one has done particularly well, scoring higher than 99% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 273,397 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 99% of its contemporaries.
We're also able to compare this research output to 119 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 98% of its contemporaries.