| Title |
The relevance of coagulation factor X protection of adenoviruses in human sera
|
|---|---|
| Published in |
Gene Therapy, March 2016
|
| DOI | 10.1038/gt.2016.32 |
| Pubmed ID | |
| Authors |
M R Duffy, A Doszpoly, G Turner, S A Nicklin, A H Baker |
| Abstract |
Intravenous delivery of adenoviruses is the optimal route for many gene therapy applications. Once in the blood, coagulation factor X (FX) binds to the adenovirus capsid and protects the virion from natural antibody and classical complement-mediated neutralisation in mice. However, to date no studies have examined the relevance of this FX/viral immune protective mechanism in human samples. In this study we assessed the effects of blocking FX on adenovirus type 5 (Ad5) activity in the presence of human serum. FX prevented human IgM binding directly to the virus. In individual human sera samples (n=25), approximately half of those screened inhibited adenovirus transduction only when the Ad5:FX interaction was blocked, demonstrating that FX protected the virus from neutralising components in a large proportion of human sera. In contrast, the remainder of sera tested had no inhibitory effects on Ad5 transduction and FX armament was not required for effective gene transfer. In human sera in which FX had a protective role, Ad5 induced lower levels of complement activation in the presence of FX. We therefore demonstrate for the first time the importance of Ad:FX protection in human samples and highlight subject variability and species-specific differences as key considerations for adenoviral gene therapy.Gene Therapy accepted article preview online, 25 March 2016. doi:10.1038/gt.2016.32. |
X Demographics
The data shown below were collected from the profiles of 14 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 14% |
| India | 1 | 7% |
| Brazil | 1 | 7% |
| United Kingdom | 1 | 7% |
| Czechia | 1 | 7% |
| Unknown | 8 | 57% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 9 | 64% |
| Practitioners (doctors, other healthcare professionals) | 2 | 14% |
| Science communicators (journalists, bloggers, editors) | 1 | 7% |
| Scientists | 1 | 7% |
| Unknown | 1 | 7% |
Mendeley readers
The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 28 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 6 | 21% |
| Student > Ph. D. Student | 6 | 21% |
| Student > Master | 5 | 18% |
| Researcher | 4 | 14% |
| Professor | 1 | 4% |
| Other | 3 | 11% |
| Unknown | 3 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 11 | 39% |
| Agricultural and Biological Sciences | 5 | 18% |
| Immunology and Microbiology | 4 | 14% |
| Social Sciences | 1 | 4% |
| Medicine and Dentistry | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 6 | 21% |
Attention Score in Context
This research output has an Altmetric Attention Score of 25. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 September 2023.
All research outputs
#1,576,051
of 25,736,439 outputs
Outputs from Gene Therapy
#81
of 3,091 outputs
Outputs of similar age
#25,803
of 315,627 outputs
Outputs of similar age from Gene Therapy
#1
of 24 outputs
Altmetric has tracked 25,736,439 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 93rd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,091 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 8.3. This one has done particularly well, scoring higher than 97% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 315,627 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 91% of its contemporaries.
We're also able to compare this research output to 24 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 95% of its contemporaries.