@PracheeAC @HenriquesLab also this one https://t.co/jWfworaSm1
@JFattaccioli @myosinactncrazy Yes exactly! This one: https://t.co/86MbYgPS96
Thanks for pointing that out - here's the paper for others who may have missed it: Flores et al. (2019)Lifeact-TagGFP2 alters F-actin organization, cellular morphology and biophysical behaviour.Sci Rep 9, 3241 https://t.co/3IleJZnPAx
@LaHauke @Avatarspirit27 @zeiss_micro a few links in various experimental systems: https://t.co/FMDhblT6iG https://t.co/8elMNRs2QG https://t.co/YsTi2FxT6d https://t.co/1MtucB8Gxw https://t.co/TfD9SrBX5m
RT @BioTai1: Oops... https://t.co/PhbAyojDhe
RT @Alanacowell: Lifeact-GFP alters F-actin organization, cellular morphology and biophysical behaviour https://t.co/kaLgES3iJZ
RT @akankshi: I am not surprised! Moreso, because its hard to get a stable line in fish. Lifeact-GFP alters F-actin organization, cellular…
RT @akankshi: I am not surprised! Moreso, because its hard to get a stable line in fish. Lifeact-GFP alters F-actin organization, cellular…
RT @joachimgoedhart: Note that the recent paper reporting effects of Lifeact-GFP uses a poorly characterized GFP variant: TagGFP2. Every ef…
Sci Rep: Lifeact-GFP alters F-actin organization, cellular morphology and biophysical behaviour https://t.co/4cl4MY5Pkv
RT @joachimgoedhart: Note that the recent paper reporting effects of Lifeact-GFP uses a poorly characterized GFP variant: TagGFP2. Every ef…
RT @joachimgoedhart: Note that the recent paper reporting effects of Lifeact-GFP uses a poorly characterized GFP variant: TagGFP2. Every ef…
RT @joachimgoedhart: Note that the recent paper reporting effects of Lifeact-GFP uses a poorly characterized GFP variant: TagGFP2. Every ef…
@JorgeBarbazan Yes, #LifeAct expressing #DendriticCells, maybe with so high expression the cell coming from the right went crazy... https://t.co/LL2FiZCBSR
RT @Alanacowell: Lifeact-GFP alters F-actin organization, cellular morphology and biophysical behaviour https://t.co/kaLgES3iJZ
RT @Alanacowell: Lifeact-GFP alters F-actin organization, cellular morphology and biophysical behaviour https://t.co/kaLgES3iJZ
Mmm.
RT @joachimgoedhart: Note that the recent paper reporting effects of Lifeact-GFP uses a poorly characterized GFP variant: TagGFP2. Every ef…
RT @robinsall: But my friends, what did you expect? All probes affect their targets, especially at the microscale. Still, glad it's been…
RT @joachimgoedhart: Note that the recent paper reporting effects of Lifeact-GFP uses a poorly characterized GFP variant: TagGFP2. Every ef…
RT @joachimgoedhart: Note that the recent paper reporting effects of Lifeact-GFP uses a poorly characterized GFP variant: TagGFP2. Every ef…
RT @joachimgoedhart: Note that the recent paper reporting effects of Lifeact-GFP uses a poorly characterized GFP variant: TagGFP2. Every ef…
RT @CuriousMat_mx: Thanks for this important precision @joachimgoedhart, this had not been discussed I think. It's been a paper everyone ha…
Thanks for this important precision @joachimgoedhart, this had not been discussed I think. It's been a paper everyone has been talking about!
Interesting side note by @joachimgoedhart on a recent publication that reports artefacts of LifeAct on cellular behaviour.
RT @joachimgoedhart: Note that the recent paper reporting effects of Lifeact-GFP uses a poorly characterized GFP variant: TagGFP2. Every ef…
If there is anyone I trust to give the straight scoop on FPs, it’s @joachimgoedhart
RT @pj_saez: A must read for #cellbiology Paper @SciReports shows how LifeAct reduces cofilin binding to actin, impacting #actin dynamics,…
RT @joachimgoedhart: Note that the recent paper reporting effects of Lifeact-GFP uses a poorly characterized GFP variant: TagGFP2. Every ef…
RT @NikonInst: Uh oh, new research shows that the commonly used actin reporter Lifeact-GFP alters the organization of the cytoskeleton, res…
RT @joachimgoedhart: Note that the recent paper reporting effects of Lifeact-GFP uses a poorly characterized GFP variant: TagGFP2. Every ef…
RT @joachimgoedhart: Note that the recent paper reporting effects of Lifeact-GFP uses a poorly characterized GFP variant: TagGFP2. Every ef…
RT @NikonInst: Uh oh, new research shows that the commonly used actin reporter Lifeact-GFP alters the organization of the cytoskeleton, res…
RT @NikonInst: Uh oh, new research shows that the commonly used actin reporter Lifeact-GFP alters the organization of the cytoskeleton, res…
RT @NikonInst: Uh oh, new research shows that the commonly used actin reporter Lifeact-GFP alters the organization of the cytoskeleton, res…
Uh oh, new research shows that the commonly used actin reporter Lifeact-GFP alters the organization of the cytoskeleton, resulting in several abnormalities. https://t.co/Nx4oS19tMN https://t.co/M0TETwwtvm
RT @SeptinLab: Everyone's freaking out about this, but Pollard's group has already shown this in 2016! mCherry-LifeAct is better behaved th…
RT @SophieMartinLab: Any tag or probe is likely to have an effect on cellular physiology. It does not mean it is useless, just should be us…
RT @joachimgoedhart: Note that the recent paper reporting effects of Lifeact-GFP uses a poorly characterized GFP variant: TagGFP2. Every ef…
RT @boehninglab: Reminder that all biosensors affect cellular physiology, including the process which you are trying to monitor. https://t.…
RT @SophieMartinLab: Any tag or probe is likely to have an effect on cellular physiology. It does not mean it is useless, just should be us…
RT @pj_saez: A must read for #cellbiology Paper @SciReports shows how LifeAct reduces cofilin binding to actin, impacting #actin dynamics,…
RT @joachimgoedhart: Note that the recent paper reporting effects of Lifeact-GFP uses a poorly characterized GFP variant: TagGFP2. Every ef…
RT @SeptinLab: Everyone's freaking out about this, but Pollard's group has already shown this in 2016! mCherry-LifeAct is better behaved th…
RT @pj_saez: A must read for #cellbiology Paper @SciReports shows how LifeAct reduces cofilin binding to actin, impacting #actin dynamics,…
RT @SeptinLab: Everyone's freaking out about this, but Pollard's group has already shown this in 2016! mCherry-LifeAct is better behaved th…
RT @pj_saez: A must read for #cellbiology Paper @SciReports shows how LifeAct reduces cofilin binding to actin, impacting #actin dynamics,…
RT @joachimgoedhart: Note that the recent paper reporting effects of Lifeact-GFP uses a poorly characterized GFP variant: TagGFP2. Every ef…
@Invadosome
RT @Alanacowell: Lifeact-GFP alters F-actin organization, cellular morphology and biophysical behaviour https://t.co/kaLgES3iJZ
RT @SeptinLab: Everyone's freaking out about this, but Pollard's group has already shown this in 2016! mCherry-LifeAct is better behaved th…
RT @joachimgoedhart: Note that the recent paper reporting effects of Lifeact-GFP uses a poorly characterized GFP variant: TagGFP2. Every ef…
RT @SeptinLab: Everyone's freaking out about this, but Pollard's group has already shown this in 2016! mCherry-LifeAct is better behaved th…
RT @pj_saez: A must read for #cellbiology Paper @SciReports shows how LifeAct reduces cofilin binding to actin, impacting #actin dynamics,…
You have a point @joachimgoedhart! Or at least multiple well known FPs should be tested before reaching a very strong conclusion.
RT @joachimgoedhart: Note that the recent paper reporting effects of Lifeact-GFP uses a poorly characterized GFP variant: TagGFP2. Every ef…
RT @Alanacowell: Lifeact-GFP alters F-actin organization, cellular morphology and biophysical behaviour https://t.co/kaLgES3iJZ
RT @joachimgoedhart: Note that the recent paper reporting effects of Lifeact-GFP uses a poorly characterized GFP variant: TagGFP2. Every ef…
RT @joachimgoedhart: Note that the recent paper reporting effects of Lifeact-GFP uses a poorly characterized GFP variant: TagGFP2. Every ef…
RT @joachimgoedhart: Note that the recent paper reporting effects of Lifeact-GFP uses a poorly characterized GFP variant: TagGFP2. Every ef…
RT @MAngieSerrano: Lifeact-GFP alters F-actin organization, cellular morphology and biophysical behaviour | Scientific Reports https://t.co…
RT @joachimgoedhart: Note that the recent paper reporting effects of Lifeact-GFP uses a poorly characterized GFP variant: TagGFP2. Every ef…
RT @joachimgoedhart: Note that the recent paper reporting effects of Lifeact-GFP uses a poorly characterized GFP variant: TagGFP2. Every ef…
I'm still trying to get the hang of Twitter. What to do w separate threads on the same topic (SciReport on LifeAct-GFP https://t.co/yvwblzbzUw)? Ea thread has good pts but also misses important pts. Can they be brought together? On which do I chime in? #so
RT @SeptinLab: Everyone's freaking out about this, but Pollard's group has already shown this in 2016! mCherry-LifeAct is better behaved th…
RT @joachimgoedhart: Note that the recent paper reporting effects of Lifeact-GFP uses a poorly characterized GFP variant: TagGFP2. Every ef…
RT @SeptinLab: Everyone's freaking out about this, but Pollard's group has already shown this in 2016! mCherry-LifeAct is better behaved th…
RT @joachimgoedhart: Note that the recent paper reporting effects of Lifeact-GFP uses a poorly characterized GFP variant: TagGFP2. Every ef…
RT @akankshi: I am not surprised! Moreso, because its hard to get a stable line in fish. Lifeact-GFP alters F-actin organization, cellular…
RT @joachimgoedhart: Note that the recent paper reporting effects of Lifeact-GFP uses a poorly characterized GFP variant: TagGFP2. Every ef…
RT @Alanacowell: Lifeact-GFP alters F-actin organization, cellular morphology and biophysical behaviour https://t.co/kaLgES3iJZ
RT @Alanacowell: Lifeact-GFP alters F-actin organization, cellular morphology and biophysical behaviour https://t.co/kaLgES3iJZ
RT @joachimgoedhart: Note that the recent paper reporting effects of Lifeact-GFP uses a poorly characterized GFP variant: TagGFP2. Every ef…
RT @joachimgoedhart: Note that the recent paper reporting effects of Lifeact-GFP uses a poorly characterized GFP variant: TagGFP2. Every ef…
RT @P_M_Mueller: Interesting discussion on LifeAct going on: Overexpression of ANY marker or probe will probably change the dynamics of a c…
RT @Alanacowell: Lifeact-GFP alters F-actin organization, cellular morphology and biophysical behaviour https://t.co/kaLgES3iJZ
RT @akankshi: I am not surprised! Moreso, because its hard to get a stable line in fish. Lifeact-GFP alters F-actin organization, cellular…
RT @joachimgoedhart: Note that the recent paper reporting effects of Lifeact-GFP uses a poorly characterized GFP variant: TagGFP2. Every ef…
RT @Alanacowell: Lifeact-GFP alters F-actin organization, cellular morphology and biophysical behaviour https://t.co/kaLgES3iJZ
Important note:
RT @joachimgoedhart: Note that the recent paper reporting effects of Lifeact-GFP uses a poorly characterized GFP variant: TagGFP2. Every ef…
RT @joachimgoedhart: Note that the recent paper reporting effects of Lifeact-GFP uses a poorly characterized GFP variant: TagGFP2. Every ef…
RT @TalleyJLambert: Couldn’t agree more https://t.co/zCLIayUk1r
RT @joachimgoedhart: Note that the recent paper reporting effects of Lifeact-GFP uses a poorly characterized GFP variant: TagGFP2. Every ef…
RT @joachimgoedhart: Note that the recent paper reporting effects of Lifeact-GFP uses a poorly characterized GFP variant: TagGFP2. Every ef…
Duhhh!
RT @boehninglab: Reminder that all biosensors affect cellular physiology, including the process which you are trying to monitor. https://t.…
RT @joachimgoedhart: Note that the recent paper reporting effects of Lifeact-GFP uses a poorly characterized GFP variant: TagGFP2. Every ef…
RT @SeptinLab: Everyone's freaking out about this, but Pollard's group has already shown this in 2016! mCherry-LifeAct is better behaved th…
RT @joachimgoedhart: Note that the recent paper reporting effects of Lifeact-GFP uses a poorly characterized GFP variant: TagGFP2. Every ef…
RT @SeptinLab: Everyone's freaking out about this, but Pollard's group has already shown this in 2016! mCherry-LifeAct is better behaved th…
RT @joachimgoedhart: Note that the recent paper reporting effects of Lifeact-GFP uses a poorly characterized GFP variant: TagGFP2. Every ef…
Couldn’t agree more
RT @joachimgoedhart: Note that the recent paper reporting effects of Lifeact-GFP uses a poorly characterized GFP variant: TagGFP2. Every ef…
Note that the recent paper reporting effects of Lifeact-GFP uses a poorly characterized GFP variant: TagGFP2. Every effect that is observed may well be due to the fluorescent protein. https://t.co/4PMH8d91xV
RT @pj_saez: A must read for #cellbiology Paper @SciReports shows how LifeAct reduces cofilin binding to actin, impacting #actin dynamics,…
RT @boehninglab: Reminder that all biosensors affect cellular physiology, including the process which you are trying to monitor. https://t.…
RT @Alanacowell: Lifeact-GFP alters F-actin organization, cellular morphology and biophysical behaviour https://t.co/kaLgES3iJZ