Title |
SIMcheck: a Toolbox for Successful Super-resolution Structured Illumination Microscopy
|
---|---|
Published in |
Scientific Reports, November 2015
|
DOI | 10.1038/srep15915 |
Pubmed ID | |
Authors |
Graeme Ball, Justin Demmerle, Rainer Kaufmann, Ilan Davis, Ian M. Dobbie, Lothar Schermelleh |
Abstract |
Three-dimensional structured illumination microscopy (3D-SIM) is a versatile and accessible method for super-resolution fluorescence imaging, but generating high-quality data is challenging, particularly for non-specialist users. We present SIMcheck, a suite of ImageJ plugins enabling users to identify and avoid common problems with 3D-SIM data, and assess resolution and data quality through objective control parameters. Additionally, SIMcheck provides advanced calibration tools and utilities for common image processing tasks. This open-source software is applicable to all commercial and custom platforms, and will promote routine application of super-resolution SIM imaging in cell biology. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 3 | 23% |
United States | 2 | 15% |
France | 2 | 15% |
Portugal | 1 | 8% |
Switzerland | 1 | 8% |
Unknown | 4 | 31% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 9 | 69% |
Scientists | 3 | 23% |
Science communicators (journalists, bloggers, editors) | 1 | 8% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 3 | 1% |
Germany | 3 | 1% |
United Kingdom | 3 | 1% |
France | 2 | <1% |
Italy | 1 | <1% |
India | 1 | <1% |
Portugal | 1 | <1% |
Israel | 1 | <1% |
Czechia | 1 | <1% |
Other | 0 | 0% |
Unknown | 253 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 71 | 26% |
Researcher | 52 | 19% |
Student > Master | 25 | 9% |
Student > Bachelor | 24 | 9% |
Student > Doctoral Student | 15 | 6% |
Other | 33 | 12% |
Unknown | 49 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 58 | 22% |
Agricultural and Biological Sciences | 48 | 18% |
Physics and Astronomy | 40 | 15% |
Engineering | 24 | 9% |
Neuroscience | 14 | 5% |
Other | 36 | 13% |
Unknown | 49 | 18% |