| Title |
A dynamic three-step mechanism drives the HIV-1 pre-fusion reaction
|
|---|---|
| Published in |
Nature Structural & Molecular Biology, August 2018
|
| DOI | 10.1038/s41594-018-0113-x |
| Pubmed ID | |
| Authors |
Maro Iliopoulou, Rory Nolan, Luis Alvarez, Yasunori Watanabe, Charles A. Coomer, G. Maria Jakobsdottir, Thomas A. Bowden, Sergi Padilla-Parra |
| Abstract |
Little is known about the intermolecular dynamics and stoichiometry of the interactions of the human immunodeficiency virus type 1 (HIV-1) envelope (Env) protein with its receptors and co-receptors on the host cell surface. Here we analyze time-resolved HIV-1 Env interactions with T-cell surface glycoprotein CD4 (CD4) and C-C chemokine receptor type 5 (CCR5) or C-X-C chemokine receptor type 4 (CXCR4) on the surface of cells, by combining multicolor super-resolution localization microscopy (direct stochastic optical reconstruction microscopy) with fluorescence fluctuation spectroscopy imaging. Utilizing the primary isolate JR-FL and laboratory HXB2 strains, we reveal the time-resolved stoichiometry of CD4 and CCR5 or CXCR4 in the pre-fusion complex with HIV-1 Env. The HIV-1 Env pre-fusion dynamics for both R5- and X4-tropic strains consists of a three-step mechanism, which seems to differ in stoichiometry. Analyses with the monoclonal HIV-1-neutralizing antibody b12 indicate that the mechanism of inhibition differs between JR-FL and HXB2 Env. The molecular insights obtained here identify assemblies of HIV-1 Env with receptors and co-receptors as potential novel targets for inhibitor design. |
X Demographics
The data shown below were collected from the profiles of 17 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 3 | 18% |
| Australia | 3 | 18% |
| United Kingdom | 3 | 18% |
| United States | 1 | 6% |
| Canada | 1 | 6% |
| Unknown | 6 | 35% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 11 | 65% |
| Scientists | 4 | 24% |
| Science communicators (journalists, bloggers, editors) | 2 | 12% |
Mendeley readers
The data shown below were compiled from readership statistics for 76 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 76 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 18 | 24% |
| Student > Ph. D. Student | 15 | 20% |
| Student > Bachelor | 8 | 11% |
| Student > Master | 7 | 9% |
| Other | 7 | 9% |
| Other | 6 | 8% |
| Unknown | 15 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 28 | 37% |
| Agricultural and Biological Sciences | 11 | 14% |
| Immunology and Microbiology | 5 | 7% |
| Chemistry | 5 | 7% |
| Physics and Astronomy | 2 | 3% |
| Other | 10 | 13% |
| Unknown | 15 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 March 2020.
All research outputs
#3,631,196
of 25,773,273 outputs
Outputs from Nature Structural & Molecular Biology
#1,393
of 4,233 outputs
Outputs of similar age
#68,121
of 345,163 outputs
Outputs of similar age from Nature Structural & Molecular Biology
#28
of 53 outputs
Altmetric has tracked 25,773,273 research outputs across all sources so far. Compared to these this one has done well and is in the 85th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,233 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.7. This one has gotten more attention than average, scoring higher than 67% of its peers.
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