| Title |
Infrared nanospectroscopy characterization of oligomeric and fibrillar aggregates during amyloid formation
|
|---|---|
| Published in |
Nature Communications, July 2015
|
| DOI | 10.1038/ncomms8831 |
| Pubmed ID | |
| Authors |
F. S. Ruggeri, G. Longo, S. Faggiano, E. Lipiec, A. Pastore, G. Dietler |
| Abstract |
Amyloids are insoluble protein fibrillar aggregates. The importance of characterizing their aggregation has steadily increased because of their link to human diseases and material science applications. In particular, misfolding and aggregation of the Josephin domain of ataxin-3 is implicated in spinocerebellar ataxia-3. Infrared nanospectroscopy, simultaneously exploiting atomic force microscopy and infrared spectroscopy, can characterize at the nanoscale the conformational rearrangements of proteins during their aggregation. Here we demonstrate that we can individually characterize the oligomeric and fibrillar species formed along the amyloid aggregation. We describe their secondary structure, monitoring at the nanoscale an α-to-β transition, and couple these studies with an independent measurement of the evolution of their intrinsic stiffness. These results suggest that the aggregation of Josephin proceeds from the monomer state to the formation of spheroidal intermediates with a native structure. Only successively, these intermediates evolve into misfolded aggregates and into the final fibrils. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 25% |
| Unknown | 3 | 75% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 75% |
| Scientists | 1 | 25% |
Mendeley readers
The data shown below were compiled from readership statistics for 220 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 3 | 1% |
| Germany | 2 | <1% |
| Brazil | 1 | <1% |
| Italy | 1 | <1% |
| Spain | 1 | <1% |
| United Kingdom | 1 | <1% |
| Unknown | 211 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 70 | 32% |
| Researcher | 43 | 20% |
| Student > Master | 20 | 9% |
| Student > Bachelor | 14 | 6% |
| Student > Doctoral Student | 10 | 5% |
| Other | 29 | 13% |
| Unknown | 34 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 50 | 23% |
| Biochemistry, Genetics and Molecular Biology | 33 | 15% |
| Physics and Astronomy | 27 | 12% |
| Agricultural and Biological Sciences | 19 | 9% |
| Engineering | 14 | 6% |
| Other | 33 | 15% |
| Unknown | 44 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 52. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 April 2021.
All research outputs
#685,634
of 22,818,766 outputs
Outputs from Nature Communications
#11,782
of 46,993 outputs
Outputs of similar age
#9,082
of 263,394 outputs
Outputs of similar age from Nature Communications
#155
of 796 outputs
Altmetric has tracked 22,818,766 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 96th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 46,993 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 55.7. This one has gotten more attention than average, scoring higher than 74% of its peers.
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We're also able to compare this research output to 796 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 80% of its contemporaries.