| Title |
Efficient targeted resequencing of human germline and cancer genomes by oligonucleotide-selective sequencing
|
|---|---|
| Published in |
Nature Biotechnology, October 2011
|
| DOI | 10.1038/nbt.1996 |
| Pubmed ID | |
| Authors |
Samuel Myllykangas, Jason D Buenrostro, Georges Natsoulis, John M Bell, Hanlee P Ji |
| Abstract |
We describe an approach for targeted genome resequencing, called oligonucleotide-selective sequencing (OS-Seq), in which we modify the immobilized lawn of oligonucleotide primers of a next-generation DNA sequencer to function as both a capture and sequencing substrate. We apply OS-Seq to resequence the exons of either 10 or 344 cancer genes from human DNA samples. In our assessment of capture performance, >87% of the captured sequence originated from the intended target region with sequencing coverage falling within a tenfold range for a majority of all targets. Single nucleotide variants (SNVs) called from OS-Seq data agreed with >95% of variants obtained from whole-genome sequencing of the same individual. We also demonstrate mutation discovery from a colorectal cancer tumor sample matched with normal tissue. Overall, we show the robust performance and utility of OS-Seq for the resequencing analysis of human germline and cancer genomes. |
X Demographics
The data shown below were collected from the profiles of 6 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 17% |
| Unknown | 5 | 83% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 67% |
| Science communicators (journalists, bloggers, editors) | 1 | 17% |
| Practitioners (doctors, other healthcare professionals) | 1 | 17% |
Mendeley readers
The data shown below were compiled from readership statistics for 213 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 8 | 4% |
| Germany | 4 | 2% |
| United States | 4 | 2% |
| China | 3 | 1% |
| Israel | 1 | <1% |
| India | 1 | <1% |
| Brazil | 1 | <1% |
| Canada | 1 | <1% |
| France | 1 | <1% |
| Other | 2 | <1% |
| Unknown | 187 | 88% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 90 | 42% |
| Student > Ph. D. Student | 42 | 20% |
| Other | 20 | 9% |
| Professor > Associate Professor | 16 | 8% |
| Student > Master | 9 | 4% |
| Other | 25 | 12% |
| Unknown | 11 | 5% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 121 | 57% |
| Biochemistry, Genetics and Molecular Biology | 36 | 17% |
| Medicine and Dentistry | 20 | 9% |
| Computer Science | 3 | 1% |
| Immunology and Microbiology | 3 | 1% |
| Other | 11 | 5% |
| Unknown | 19 | 9% |
Attention Score in Context
This research output has an Altmetric Attention Score of 16. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 April 2024.
All research outputs
#2,353,863
of 26,017,215 outputs
Outputs from Nature Biotechnology
#3,150
of 8,611 outputs
Outputs of similar age
#11,890
of 156,924 outputs
Outputs of similar age from Nature Biotechnology
#26
of 85 outputs
Altmetric has tracked 26,017,215 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 90th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 8,611 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 44.6. This one has gotten more attention than average, scoring higher than 63% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 156,924 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 91% of its contemporaries.
We're also able to compare this research output to 85 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 69% of its contemporaries.