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Somatic mutations in leukocytes infiltrating primary breast cancers

Overview of attention for article published in npj Breast Cancer, June 2015
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (83rd percentile)
  • Average Attention Score compared to outputs of the same age and source

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4 X users
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2 patents
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6 Facebook pages

Citations

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30 Dimensions

Readers on

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53 Mendeley
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1 CiteULike
Title
Somatic mutations in leukocytes infiltrating primary breast cancers
Published in
npj Breast Cancer, June 2015
DOI 10.1038/npjbcancer.2015.5
Pubmed ID
Authors

Maria Kleppe, Elizabeth Comen, Hannah Y Wen, Lennart Bastian, Brian Blum, Franck T Rapaport, Matthew Keller, Zvika Granot, Nicolas Socci, Agnès Viale, Daoqi You, Robert Benezra, Britta Weigelt, Edi Brogi, Michael F Berger, Jorge S Reis-Filho, Ross L Levine, Larry Norton

Abstract

Malignant transformation requires the interaction of cancer cells with their microenvironment, including infiltrating leukocytes. However, somatic mutational studies have focused on alterations in cancer cells, assuming that the microenvironment is genetically normal. Because we hypothesized that this might not be a valid assumption, we performed exome sequencing and targeted sequencing to investigate for the presence of pathogenic mutations in tumor-associated leukocytes in breast cancers. We used targeted sequencing and exome sequencing to evaluate the presence of mutations in sorted tumor-infiltrating CD45-positive cells from primary untreated breast cancers. We used high-depth sequencing to determine the presence/absence of the mutations we identified in breast cancer-infiltrating leukocytes in purified tumor cells and in circulating blood cells. Capture-based sequencing of 15 paired tumor-infiltrating leukocytes and matched germline DNA identified variants in known cancer genes in all 15 primary breast cancer patients in our cohort. We validated the presence of mutations identified by targeted sequencing in infiltrating leukocytes through orthogonal exome sequencing. Ten patients harbored alterations previously reported as somatically acquired variants, including in known leukemia genes (DNTM3A, TET2, and BCOR). One of the mutations observed in the tumor-infiltrating leukocytes was also detected in the circulating leukocytes of the same patients at a lower allele frequency than observed in the tumor-infiltrating cells. Here we show that somatic mutations, including mutations in known cancer genes, are present in the leukocytes infiltrating a subset of primary breast cancers. This observation allows for the possibility that the cancer cells interact with mutant infiltrating leukocytes, which has many potential clinical implications.

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X Demographics

The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 53 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
France 1 2%
Unknown 52 98%

Demographic breakdown

Readers by professional status Count As %
Researcher 15 28%
Student > Ph. D. Student 11 21%
Student > Bachelor 4 8%
Professor > Associate Professor 4 8%
Student > Doctoral Student 3 6%
Other 6 11%
Unknown 10 19%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 16 30%
Agricultural and Biological Sciences 10 19%
Medicine and Dentistry 9 17%
Immunology and Microbiology 2 4%
Neuroscience 2 4%
Other 2 4%
Unknown 12 23%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 October 2020.
All research outputs
#3,120,686
of 22,813,792 outputs
Outputs from npj Breast Cancer
#178
of 501 outputs
Outputs of similar age
#42,223
of 266,642 outputs
Outputs of similar age from npj Breast Cancer
#3
of 6 outputs
Altmetric has tracked 22,813,792 research outputs across all sources so far. Compared to these this one has done well and is in the 86th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 501 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 25.0. This one has gotten more attention than average, scoring higher than 63% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 266,642 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 83% of its contemporaries.
We're also able to compare this research output to 6 others from the same source and published within six weeks on either side of this one. This one has scored higher than 3 of them.