| Title |
Simultaneous Amplification and Detection of Specific DNA Sequences
|
|---|---|
| Published in |
Biotechnology, April 1992
|
| DOI | 10.1038/nbt0492-413 |
| Pubmed ID | |
| Authors |
Russell Higuchi, Gavin Dollinger, P. Sean Walsh, Robert Griffith |
| Abstract |
We have enhanced the polymerase chain reaction (PCR) such that specific DNA sequences can be detected without opening the reaction tube. This enhancement requires the addition of ethidium bromide (EtBr) to a PCR. Since the fluorescence of EtBr increases in the presence of double-stranded (ds) DNA an increase in fluorescence in such a PCR indicates a positive amplification, which can be easily monitored externally. In fact, amplification can be continuously monitored in order to follow its progress. The ability to simultaneously amplify specific DNA sequences and detect the product of the amplification both simplifies and improves PCR and may facilitate its automation and more widespread use in the clinic or in other situations requiring high sample throughput. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 33% |
| Unknown | 2 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 67% |
| Scientists | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 796 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 4 | <1% |
| United Kingdom | 4 | <1% |
| Austria | 2 | <1% |
| Brazil | 2 | <1% |
| Mexico | 2 | <1% |
| Malaysia | 2 | <1% |
| Canada | 2 | <1% |
| Taiwan | 1 | <1% |
| Germany | 1 | <1% |
| Other | 4 | <1% |
| Unknown | 772 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 143 | 18% |
| Student > Master | 131 | 16% |
| Student > Bachelor | 114 | 14% |
| Researcher | 80 | 10% |
| Student > Doctoral Student | 54 | 7% |
| Other | 109 | 14% |
| Unknown | 165 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 227 | 29% |
| Biochemistry, Genetics and Molecular Biology | 173 | 22% |
| Medicine and Dentistry | 61 | 8% |
| Engineering | 23 | 3% |
| Chemistry | 19 | 2% |
| Other | 98 | 12% |
| Unknown | 195 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 32. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 April 2024.
All research outputs
#1,263,825
of 25,559,053 outputs
Outputs from Biotechnology
#5
of 494 outputs
Outputs of similar age
#183
of 18,226 outputs
Outputs of similar age from Biotechnology
#1
of 18 outputs
Altmetric has tracked 25,559,053 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 95th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 494 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.6. This one has done particularly well, scoring higher than 99% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 18,226 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 99% of its contemporaries.
We're also able to compare this research output to 18 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 99% of its contemporaries.