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Rapid reverse genetic screening using CRISPR in zebrafish

Overview of attention for article published in Nature Methods, April 2015
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (88th percentile)
  • Above-average Attention Score compared to outputs of the same age and source (52nd percentile)

Mentioned by

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1 news outlet
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9 X users
facebook
1 Facebook page

Citations

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318 Dimensions

Readers on

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583 Mendeley
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2 CiteULike
Title
Rapid reverse genetic screening using CRISPR in zebrafish
Published in
Nature Methods, April 2015
DOI 10.1038/nmeth.3360
Pubmed ID
Authors

Arish N Shah, Crystal F Davey, Alex C Whitebirch, Adam C Miller, Cecilia B Moens

Abstract

Identifying genes involved in biological processes is critical for understanding the molecular building blocks of life. We used engineered CRISPR (clustered regularly interspaced short palindromic repeats) to efficiently mutate specific loci in zebrafish (Danio rerio) and screen for genes involved in vertebrate biological processes. We found that increasing CRISPR efficiency by injecting optimized amounts of Cas9-encoding mRNA and multiplexing single guide RNAs (sgRNAs) allowed for phenocopy of known mutants across many phenotypes in embryos. We performed a proof-of-concept screen in which we used intersecting, multiplexed pool injections to examine 48 loci and identified two new genes involved in electrical-synapse formation. By deep sequencing target loci, we found that 90% of the genes were effectively screened. We conclude that CRISPR can be used as a powerful reverse genetic screening strategy in vivo in a vertebrate system.

X Demographics

X Demographics

The data shown below were collected from the profiles of 9 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 583 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 6 1%
Portugal 2 <1%
Canada 2 <1%
United States 2 <1%
Australia 2 <1%
Brazil 2 <1%
Indonesia 1 <1%
Chile 1 <1%
Austria 1 <1%
Other 5 <1%
Unknown 559 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 132 23%
Researcher 97 17%
Student > Bachelor 83 14%
Student > Master 74 13%
Student > Doctoral Student 33 6%
Other 84 14%
Unknown 80 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 222 38%
Biochemistry, Genetics and Molecular Biology 158 27%
Neuroscience 40 7%
Medicine and Dentistry 29 5%
Engineering 9 2%
Other 37 6%
Unknown 88 15%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 14. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 March 2021.
All research outputs
#2,161,346
of 22,799,071 outputs
Outputs from Nature Methods
#2,171
of 4,909 outputs
Outputs of similar age
#29,720
of 264,583 outputs
Outputs of similar age from Nature Methods
#51
of 107 outputs
Altmetric has tracked 22,799,071 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 90th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,909 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 34.6. This one has gotten more attention than average, scoring higher than 55% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 264,583 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 88% of its contemporaries.
We're also able to compare this research output to 107 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 52% of its contemporaries.