| Title |
DNA stretching by bacterial initiators promotes replication origin opening
|
|---|---|
| Published in |
Nature, October 2011
|
| DOI | 10.1038/nature10455 |
| Pubmed ID | |
| Authors |
Karl E. Duderstadt, Kevin Chuang, James M. Berger |
| Abstract |
Many replication initiators form higher-order oligomers that process host replication origins to promote replisome formation. In addition to dedicated duplex-DNA-binding domains, cellular initiators possess AAA+ (ATPases associated with various cellular activities) elements that drive functions ranging from protein assembly to origin recognition. In bacteria, the AAA+ domain of the initiator DnaA has been proposed to assist in single-stranded DNA formation during origin melting. Here we show crystallographically and in solution that the ATP-dependent assembly of Aquifex aeolicus DnaA into a spiral oligomer creates a continuous surface that allows successive AAA+ domains to bind and extend single-stranded DNA segments. The mechanism of binding is unexpectedly similar to that of RecA, a homologous recombination factor, but it differs in that DnaA promotes a nucleic acid conformation that prevents pairing of a complementary strand. These findings, combined with strand-displacement assays, indicate that DnaA opens replication origins by a direct ATP-dependent stretching mechanism. Comparative studies reveal notable commonalities between the approach used by DnaA to engage DNA substrates and other, nucleic-acid-dependent, AAA+ systems. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 268 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 9 | 3% |
| United Kingdom | 4 | 1% |
| Japan | 3 | 1% |
| France | 2 | <1% |
| Sweden | 1 | <1% |
| Estonia | 1 | <1% |
| Chile | 1 | <1% |
| Mexico | 1 | <1% |
| Spain | 1 | <1% |
| Other | 0 | 0% |
| Unknown | 245 | 91% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 76 | 28% |
| Researcher | 59 | 22% |
| Student > Bachelor | 27 | 10% |
| Student > Master | 24 | 9% |
| Student > Doctoral Student | 12 | 4% |
| Other | 37 | 14% |
| Unknown | 33 | 12% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 127 | 47% |
| Biochemistry, Genetics and Molecular Biology | 57 | 21% |
| Chemistry | 21 | 8% |
| Engineering | 7 | 3% |
| Physics and Astronomy | 7 | 3% |
| Other | 15 | 6% |
| Unknown | 34 | 13% |
Attention Score in Context
This research output has an Altmetric Attention Score of 17. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 June 2022.
All research outputs
#1,851,826
of 22,675,759 outputs
Outputs from Nature
#39,828
of 90,630 outputs
Outputs of similar age
#9,412
of 132,641 outputs
Outputs of similar age from Nature
#503
of 935 outputs
Altmetric has tracked 22,675,759 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 91st percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 90,630 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 99.1. This one has gotten more attention than average, scoring higher than 56% of its peers.
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