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Next generation histology methods for three-dimensional imaging of fresh and archival human brain tissues

Overview of attention for article published in Nature Communications, March 2018
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (96th percentile)
  • High Attention Score compared to outputs of the same age and source (81st percentile)

Mentioned by

news
3 news outlets
blogs
7 blogs
twitter
34 X users
facebook
4 Facebook pages
video
1 YouTube creator

Citations

dimensions_citation
101 Dimensions

Readers on

mendeley
234 Mendeley
Title
Next generation histology methods for three-dimensional imaging of fresh and archival human brain tissues
Published in
Nature Communications, March 2018
DOI 10.1038/s41467-018-03359-w
Pubmed ID
Authors

Hei Ming Lai, Alan King Lun Liu, Harry Ho Man Ng, Marc H. Goldfinger, Tsz Wing Chau, John DeFelice, Bension S. Tilley, Wai Man Wong, Wutian Wu, Steve M. Gentleman

Abstract

Modern clearing techniques for the three-dimensional (3D) visualisation of neural tissue microstructure have been very effective when used on rodent brain but very few studies have utilised them on human brain material, mainly due to the inherent difficulties in processing post-mortem tissue. Here we develop a tissue clearing solution, OPTIClear, optimised for fresh and archival human brain tissue, including formalin-fixed paraffin-embedded material. In light of practical challenges with immunostaining in tissue clearing, we adapt the use of cresyl violet for visualisation of neurons in cleared tissue, with the potential for 3D quantification in regions of interest. Furthermore, we use lipophilic tracers for tracing of neuronal processes in post-mortem tissue, enabling the study of the morphology of human dendritic spines in 3D. The development of these different strategies for human tissue clearing has wide applicability and, we hope, will provide a baseline for further technique development.

X Demographics

X Demographics

The data shown below were collected from the profiles of 34 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 234 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 234 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 43 18%
Student > Ph. D. Student 41 18%
Student > Master 24 10%
Student > Bachelor 21 9%
Student > Doctoral Student 13 6%
Other 34 15%
Unknown 58 25%
Readers by discipline Count As %
Neuroscience 56 24%
Medicine and Dentistry 24 10%
Agricultural and Biological Sciences 23 10%
Biochemistry, Genetics and Molecular Biology 16 7%
Engineering 14 6%
Other 36 15%
Unknown 65 28%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 82. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 December 2023.
All research outputs
#525,224
of 25,498,750 outputs
Outputs from Nature Communications
#8,952
of 57,362 outputs
Outputs of similar age
#12,168
of 352,087 outputs
Outputs of similar age from Nature Communications
#231
of 1,225 outputs
Altmetric has tracked 25,498,750 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 97th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 57,362 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 55.5. This one has done well, scoring higher than 84% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 352,087 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 96% of its contemporaries.
We're also able to compare this research output to 1,225 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 81% of its contemporaries.