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AlkB reverses etheno DNA lesions caused by lipid oxidation in vitro and in vivo

Overview of attention for article published in Nature Structural & Molecular Biology, October 2005
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Title
AlkB reverses etheno DNA lesions caused by lipid oxidation in vitro and in vivo
Published in
Nature Structural & Molecular Biology, October 2005
DOI 10.1038/nsmb996
Pubmed ID
Authors

James C Delaney, Lisa Smeester, Cintyu Wong, Lauren E Frick, Koli Taghizadeh, John S Wishnok, Catherine L Drennan, Leona D Samson, John M Essigmann

Abstract

Oxidative stress converts lipids into DNA-damaging agents. The genomic lesions formed include 1,N(6)-ethenoadenine (epsilonA) and 3,N(4)-ethenocytosine (epsilonC), in which two carbons of the lipid alkyl chain form an exocyclic adduct with a DNA base. Here we show that the newly characterized enzyme AlkB repairs epsilonA and epsilonC. The potent toxicity and mutagenicity of epsilonA in Escherichia coli lacking AlkB was reversed in AlkB(+) cells; AlkB also mitigated the effects of epsilonC. In vitro, AlkB cleaved the lipid-derived alkyl chain from DNA, causing epsilonA and epsilonC to revert to adenine and cytosine, respectively. Biochemically, epsilonA is epoxidized at the etheno bond. The epoxide is putatively hydrolyzed to a glycol, and the glycol moiety is released as glyoxal. These reactions show a previously unrecognized chemical versatility of AlkB. In mammals, the corresponding AlkB homologs may defend against aging, cancer and oxidative stress.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 67 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 1%
Germany 1 1%
Unknown 65 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 18 27%
Researcher 8 12%
Student > Master 8 12%
Student > Bachelor 7 10%
Professor > Associate Professor 7 10%
Other 10 15%
Unknown 9 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 20 30%
Biochemistry, Genetics and Molecular Biology 13 19%
Chemistry 12 18%
Pharmacology, Toxicology and Pharmaceutical Science 4 6%
Medicine and Dentistry 2 3%
Other 5 7%
Unknown 11 16%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 May 2012.
All research outputs
#8,534,976
of 25,374,647 outputs
Outputs from Nature Structural & Molecular Biology
#2,602
of 4,186 outputs
Outputs of similar age
#25,213
of 70,635 outputs
Outputs of similar age from Nature Structural & Molecular Biology
#11
of 27 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,186 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.5. This one is in the 20th percentile – i.e., 20% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 70,635 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 13th percentile – i.e., 13% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 27 others from the same source and published within six weeks on either side of this one. This one is in the 18th percentile – i.e., 18% of its contemporaries scored the same or lower than it.