| Title |
Localization of lipopolysaccharide from Escherichia Coli into human atherosclerotic plaque
|
|---|---|
| Published in |
Scientific Reports, February 2018
|
| DOI | 10.1038/s41598-018-22076-4 |
| Pubmed ID | |
| Authors |
Roberto Carnevale, Cristina Nocella, Vincenzo Petrozza, Vittoria Cammisotto, Luca Pacini, Veronica Sorrentino, Ombretta Martinelli, Luigi Irace, Sebastiano Sciarretta, Giacomo Frati, Daniele Pastori, Francesco Violi |
| Abstract |
Experimental studies showed that gut-derived lipopolysaccharide (LPS) is pro-atherogenic, however, its relationship with human atherosclerosis is still to be defined. We investigate if gut-derived LPS from Escherichia Coli localizes in human carotid plaque and its potential role as pro-inflammatory molecule in the atherosclerotic lesion. LPS from Escherichia Coli and Toll-like receptor 4 (TLR4) were studied in specimens from carotid and thyroid arteries of 10 patients undergoing endarterectomy and 15 controls matched for demographic and clinical characteristics. Blood LPS were significantly higher in patients compared to controls. Immunochemistry analysis revealed positivity for antibodies against LPS and TLR4 coincidentally with positivity for CD68 only in the atherosclerotic plaque of carotid arteries but not in thyroid arteries; the positivity for LPS and TLR4 was greater in the area with activated macrophages. LPS concentration similar to that detected in atherosclerotic plaque resulted in a dose-dependent TLR4-mediated Nox2 up-regulation by human monocytes. These data provide the first evidence that LPS from Escherichia Coli localizes in human plaque and may contribute to atherosclerotic damage via TLR4-mediated oxidative stress. |
X Demographics
The data shown below were collected from the profiles of 6 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 33% |
| Canada | 1 | 17% |
| Unknown | 3 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 3 | 50% |
| Members of the public | 2 | 33% |
| Practitioners (doctors, other healthcare professionals) | 1 | 17% |
Mendeley readers
The data shown below were compiled from readership statistics for 79 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 79 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 13 | 16% |
| Student > Bachelor | 12 | 15% |
| Student > Doctoral Student | 8 | 10% |
| Student > Ph. D. Student | 7 | 9% |
| Student > Master | 7 | 9% |
| Other | 15 | 19% |
| Unknown | 17 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 20 | 25% |
| Biochemistry, Genetics and Molecular Biology | 12 | 15% |
| Pharmacology, Toxicology and Pharmaceutical Science | 6 | 8% |
| Agricultural and Biological Sciences | 3 | 4% |
| Neuroscience | 2 | 3% |
| Other | 6 | 8% |
| Unknown | 30 | 38% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 May 2021.
All research outputs
#6,281,005
of 25,137,221 outputs
Outputs from Scientific Reports
#42,887
of 138,122 outputs
Outputs of similar age
#100,545
of 336,072 outputs
Outputs of similar age from Scientific Reports
#1,338
of 4,058 outputs
Altmetric has tracked 25,137,221 research outputs across all sources so far. This one has received more attention than most of these and is in the 74th percentile.
So far Altmetric has tracked 138,122 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 18.7. This one has gotten more attention than average, scoring higher than 68% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 336,072 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 69% of its contemporaries.
We're also able to compare this research output to 4,058 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.