Title |
A high-throughput optomechanical retrieval method for sequence-verified clonal DNA from the NGS platform
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Published in |
Nature Communications, February 2015
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DOI | 10.1038/ncomms7073 |
Pubmed ID | |
Authors |
Howon Lee, Hyoki Kim, Sungsik Kim, Taehoon Ryu, Hwangbeom Kim, Duhee Bang, Sunghoon Kwon |
Abstract |
Writing DNA plays a significant role in the fields of synthetic biology, functional genomics and bioengineering. DNA clones on next-generation sequencing (NGS) platforms have the potential to be a rich and cost-effective source of sequence-verified DNAs as a precursor for DNA writing. However, it is still very challenging to retrieve target clonal DNA from high-density NGS platforms. Here we propose an enabling technology called 'Sniper Cloning' that enables the precise mapping of target clone features on NGS platforms and non-contact rapid retrieval of targets for the full utilization of DNA clones. By merging the three cutting-edge technologies of NGS, DNA microarray and our pulse laser retrieval system, Sniper Cloning is a week-long process that produces 5,188 error-free synthetic DNAs in a single run of NGS with a single microarray DNA pool. We believe that this technology has potential as a universal tool for DNA writing in biological sciences. |
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Geographical breakdown
Country | Count | As % |
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United States | 2 | 29% |
United Kingdom | 1 | 14% |
Netherlands | 1 | 14% |
Sweden | 1 | 14% |
Unknown | 2 | 29% |
Demographic breakdown
Type | Count | As % |
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Scientists | 5 | 71% |
Members of the public | 2 | 29% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Korea, Republic of | 1 | 1% |
Belgium | 1 | 1% |
Unknown | 70 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 23 | 32% |
Researcher | 15 | 21% |
Student > Master | 12 | 17% |
Student > Bachelor | 5 | 7% |
Other | 4 | 6% |
Other | 7 | 10% |
Unknown | 6 | 8% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 24 | 33% |
Biochemistry, Genetics and Molecular Biology | 17 | 24% |
Engineering | 10 | 14% |
Medicine and Dentistry | 3 | 4% |
Physics and Astronomy | 2 | 3% |
Other | 7 | 10% |
Unknown | 9 | 13% |