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A high-throughput optomechanical retrieval method for sequence-verified clonal DNA from the NGS platform

Overview of attention for article published in Nature Communications, February 2015
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (89th percentile)
  • Average Attention Score compared to outputs of the same age and source

Mentioned by

twitter
7 X users
patent
2 patents
facebook
1 Facebook page
wikipedia
1 Wikipedia page

Citations

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29 Dimensions

Readers on

mendeley
72 Mendeley
citeulike
1 CiteULike
Title
A high-throughput optomechanical retrieval method for sequence-verified clonal DNA from the NGS platform
Published in
Nature Communications, February 2015
DOI 10.1038/ncomms7073
Pubmed ID
Authors

Howon Lee, Hyoki Kim, Sungsik Kim, Taehoon Ryu, Hwangbeom Kim, Duhee Bang, Sunghoon Kwon

Abstract

Writing DNA plays a significant role in the fields of synthetic biology, functional genomics and bioengineering. DNA clones on next-generation sequencing (NGS) platforms have the potential to be a rich and cost-effective source of sequence-verified DNAs as a precursor for DNA writing. However, it is still very challenging to retrieve target clonal DNA from high-density NGS platforms. Here we propose an enabling technology called 'Sniper Cloning' that enables the precise mapping of target clone features on NGS platforms and non-contact rapid retrieval of targets for the full utilization of DNA clones. By merging the three cutting-edge technologies of NGS, DNA microarray and our pulse laser retrieval system, Sniper Cloning is a week-long process that produces 5,188 error-free synthetic DNAs in a single run of NGS with a single microarray DNA pool. We believe that this technology has potential as a universal tool for DNA writing in biological sciences.

X Demographics

X Demographics

The data shown below were collected from the profiles of 7 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 72 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Korea, Republic of 1 1%
Belgium 1 1%
Unknown 70 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 23 32%
Researcher 15 21%
Student > Master 12 17%
Student > Bachelor 5 7%
Other 4 6%
Other 7 10%
Unknown 6 8%
Readers by discipline Count As %
Agricultural and Biological Sciences 24 33%
Biochemistry, Genetics and Molecular Biology 17 24%
Engineering 10 14%
Medicine and Dentistry 3 4%
Physics and Astronomy 2 3%
Other 7 10%
Unknown 9 13%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 13. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 August 2022.
All research outputs
#2,651,918
of 24,323,943 outputs
Outputs from Nature Communications
#28,663
of 51,949 outputs
Outputs of similar age
#38,076
of 360,545 outputs
Outputs of similar age from Nature Communications
#332
of 667 outputs
Altmetric has tracked 24,323,943 research outputs across all sources so far. Compared to these this one has done well and is in the 89th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 51,949 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 56.2. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 360,545 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 89% of its contemporaries.
We're also able to compare this research output to 667 others from the same source and published within six weeks on either side of this one. This one is in the 49th percentile – i.e., 49% of its contemporaries scored the same or lower than it.