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Architecture of a channel-forming O-antigen polysaccharide ABC transporter

Overview of attention for article published in Nature, January 2018
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (92nd percentile)

Mentioned by

52 tweeters
1 Facebook page
1 research highlight platform


54 Dimensions

Readers on

116 Mendeley
Architecture of a channel-forming O-antigen polysaccharide ABC transporter
Published in
Nature, January 2018
DOI 10.1038/nature25190
Pubmed ID

Yunchen Bi, Evan Mann, Chris Whitfield, Jochen Zimmer


O-antigens are cell surface polysaccharides of many Gram-negative pathogens that aid in escaping innate immune responses. A widespread O-antigen biosynthesis mechanism involves the synthesis of the lipid-anchored polymer on the cytosolic face of the inner membrane, followed by transport to the periplasmic side where it is ligated to the lipid A core to complete a lipopolysaccharide molecule. In this pathway, transport to the periplasm is mediated by an ATP-binding cassette (ABC) transporter, called Wzm-Wzt. Here we present the crystal structure of the Wzm-Wzt homologue from Aquifex aeolicus in an open conformation. The transporter forms a transmembrane channel that is sufficiently wide to accommodate a linear polysaccharide. Its nucleotide-binding domain and a periplasmic extension form 'gate helices' at the cytosolic and periplasmic membrane interfaces that probably serve as substrate entry and exit points. Site-directed mutagenesis of the gates impairs in vivo O-antigen secretion in the Escherichia coli prototype. Combined with a closed structure of the isolated nucleotide-binding domains, our structural and functional analyses suggest a processive O-antigen translocation mechanism, which stands in contrast to the classical alternating access mechanism of ABC transporters.

Twitter Demographics

The data shown below were collected from the profiles of 52 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 116 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 116 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 24 21%
Researcher 24 21%
Student > Bachelor 16 14%
Student > Master 9 8%
Professor > Associate Professor 6 5%
Other 17 15%
Unknown 20 17%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 36 31%
Agricultural and Biological Sciences 30 26%
Chemistry 8 7%
Immunology and Microbiology 6 5%
Medicine and Dentistry 5 4%
Other 9 8%
Unknown 22 19%

Attention Score in Context

This research output has an Altmetric Attention Score of 26. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 January 2018.
All research outputs
of 18,083,481 outputs
Outputs from Nature
of 81,014 outputs
Outputs of similar age
of 418,742 outputs
Outputs of similar age from Nature
of 773 outputs
Altmetric has tracked 18,083,481 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 94th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 81,014 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 91.8. This one has gotten more attention than average, scoring higher than 63% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 418,742 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 92% of its contemporaries.
We're also able to compare this research output to 773 others from the same source and published within six weeks on either side of this one. This one is in the 21st percentile – i.e., 21% of its contemporaries scored the same or lower than it.