Title |
Integrative genomic and transcriptomic analysis of leiomyosarcoma
|
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Published in |
Nature Communications, January 2018
|
DOI | 10.1038/s41467-017-02602-0 |
Pubmed ID | |
Authors |
Priya Chudasama, Sadaf S. Mughal, Mathijs A. Sanders, Daniel Hübschmann, Inn Chung, Katharina I. Deeg, Siao-Han Wong, Sophie Rabe, Mario Hlevnjak, Marc Zapatka, Aurélie Ernst, Kortine Kleinheinz, Matthias Schlesner, Lina Sieverling, Barbara Klink, Evelin Schröck, Remco M. Hoogenboezem, Bernd Kasper, Christoph E. Heilig, Gerlinde Egerer, Stephan Wolf, Christof von Kalle, Roland Eils, Albrecht Stenzinger, Wilko Weichert, Hanno Glimm, Stefan Gröschel, Hans-Georg Kopp, Georg Omlor, Burkhard Lehner, Sebastian Bauer, Simon Schimmack, Alexis Ulrich, Gunhild Mechtersheimer, Karsten Rippe, Benedikt Brors, Barbara Hutter, Marcus Renner, Peter Hohenberger, Claudia Scholl, Stefan Fröhling |
Abstract |
Leiomyosarcoma (LMS) is an aggressive mesenchymal malignancy with few therapeutic options. The mechanisms underlying LMS development, including clinically actionable genetic vulnerabilities, are largely unknown. Here we show, using whole-exome and transcriptome sequencing, that LMS tumors are characterized by substantial mutational heterogeneity, near-universal inactivation of TP53 and RB1, widespread DNA copy number alterations including chromothripsis, and frequent whole-genome duplication. Furthermore, we detect alternative telomere lengthening in 78% of cases and identify recurrent alterations in telomere maintenance genes such as ATRX, RBL2, and SP100, providing insight into the genetic basis of this mechanism. Finally, most tumors display hallmarks of "BRCAness", including alterations in homologous recombination DNA repair genes, multiple structural rearrangements, and enrichment of specific mutational signatures, and cultured LMS cells are sensitive towards olaparib and cisplatin. This comprehensive study of LMS genomics has uncovered key biological features that may inform future experimental research and enable the design of novel therapies. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 4 | 27% |
Germany | 2 | 13% |
Netherlands | 1 | 7% |
United Kingdom | 1 | 7% |
Unknown | 7 | 47% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 7 | 47% |
Members of the public | 7 | 47% |
Science communicators (journalists, bloggers, editors) | 1 | 7% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 195 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 32 | 16% |
Student > Master | 22 | 11% |
Student > Ph. D. Student | 21 | 11% |
Other | 16 | 8% |
Student > Bachelor | 16 | 8% |
Other | 29 | 15% |
Unknown | 59 | 30% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 44 | 23% |
Medicine and Dentistry | 44 | 23% |
Agricultural and Biological Sciences | 22 | 11% |
Computer Science | 4 | 2% |
Chemistry | 3 | 2% |
Other | 12 | 6% |
Unknown | 66 | 34% |