| Title |
Multiplexed 3D super-resolution imaging of whole cells using spinning disk confocal microscopy and DNA-PAINT
|
|---|---|
| Published in |
Nature Communications, December 2017
|
| DOI | 10.1038/s41467-017-02028-8 |
| Pubmed ID | |
| Authors |
Florian Schueder, Juanita Lara-Gutiérrez, Brian J. Beliveau, Sinem K. Saka, Hiroshi M. Sasaki, Johannes B. Woehrstein, Maximilian T. Strauss, Heinrich Grabmayr, Peng Yin, Ralf Jungmann |
| Abstract |
Single-molecule localization microscopy (SMLM) can visualize biological targets on the nanoscale, but complex hardware is required to perform SMLM in thick samples. Here, we combine 3D DNA points accumulation for imaging in nanoscale topography (DNA-PAINT) with spinning disk confocal (SDC) hardware to overcome this limitation. We assay our achievable resolution with two- and three-dimensional DNA origami structures and demonstrate the general applicability by imaging a large variety of cellular targets including proteins, DNA and RNA deep in cells. We achieve multiplexed 3D super-resolution imaging at sample depths up to ~10 µm with up to 20 nm planar and 80 nm axial resolution, now enabling DNA-based super-resolution microscopy in whole cells using standard instrumentation. |
X Demographics
The data shown below were collected from the profiles of 61 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 9 | 15% |
| United Kingdom | 5 | 8% |
| Japan | 4 | 7% |
| Germany | 4 | 7% |
| Canada | 2 | 3% |
| France | 2 | 3% |
| Switzerland | 2 | 3% |
| Spain | 2 | 3% |
| Hong Kong | 1 | 2% |
| Other | 2 | 3% |
| Unknown | 28 | 46% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 39 | 64% |
| Scientists | 21 | 34% |
| Science communicators (journalists, bloggers, editors) | 1 | 2% |
Mendeley readers
The data shown below were compiled from readership statistics for 397 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 397 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 103 | 26% |
| Researcher | 70 | 18% |
| Student > Master | 40 | 10% |
| Student > Bachelor | 25 | 6% |
| Student > Doctoral Student | 21 | 5% |
| Other | 53 | 13% |
| Unknown | 85 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 86 | 22% |
| Agricultural and Biological Sciences | 58 | 15% |
| Physics and Astronomy | 47 | 12% |
| Engineering | 35 | 9% |
| Chemistry | 28 | 7% |
| Other | 52 | 13% |
| Unknown | 91 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 113. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 July 2023.
All research outputs
#377,917
of 25,721,020 outputs
Outputs from Nature Communications
#5,977
of 58,206 outputs
Outputs of similar age
#8,324
of 446,017 outputs
Outputs of similar age from Nature Communications
#165
of 1,439 outputs
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So far Altmetric has tracked 58,206 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 55.5. This one has done well, scoring higher than 89% of its peers.
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We're also able to compare this research output to 1,439 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 88% of its contemporaries.