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A thermostable Cas9 with increased lifetime in human plasma

Overview of attention for article published in Nature Communications, November 2017
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (96th percentile)
  • High Attention Score compared to outputs of the same age and source (80th percentile)

Mentioned by

news
1 news outlet
blogs
1 blog
twitter
99 X users
patent
22 patents

Citations

dimensions_citation
149 Dimensions

Readers on

mendeley
275 Mendeley
Title
A thermostable Cas9 with increased lifetime in human plasma
Published in
Nature Communications, November 2017
DOI 10.1038/s41467-017-01408-4
Pubmed ID
Authors

Lucas B. Harrington, David Paez-Espino, Brett T. Staahl, Janice S. Chen, Enbo Ma, Nikos C. Kyrpides, Jennifer A. Doudna

Abstract

CRISPR-Cas9 is a powerful technology that has enabled genome editing in a wide range of species. However, the currently developed Cas9 homologs all originate from mesophilic bacteria, making them susceptible to degradation and unsuitable for applications requiring cleavage at elevated temperatures. Here, we show that the Cas9 protein from the thermophilic bacterium Geobacillus stearothermophilus (GeoCas9) catalyzes RNA-guided DNA cleavage at elevated temperatures. GeoCas9 is active at temperatures up to 70 °C, compared to 45 °C for Streptococcus pyogenes Cas9 (SpyCas9), which expands the temperature range for CRISPR-Cas9 applications. We also found that GeoCas9 is an effective tool for editing mammalian genomes when delivered as a ribonucleoprotein (RNP) complex. Together with an increased lifetime in human plasma, the thermostable GeoCas9 provides the foundation for improved RNP delivery in vivo and expands the temperature range of CRISPR-Cas9.

X Demographics

X Demographics

The data shown below were collected from the profiles of 99 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 275 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Switzerland 1 <1%
Unknown 274 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 55 20%
Researcher 48 17%
Student > Bachelor 31 11%
Student > Master 23 8%
Other 16 6%
Other 34 12%
Unknown 68 25%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 96 35%
Agricultural and Biological Sciences 54 20%
Chemistry 12 4%
Medicine and Dentistry 9 3%
Immunology and Microbiology 7 3%
Other 25 9%
Unknown 72 26%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 75. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 February 2024.
All research outputs
#575,163
of 25,584,565 outputs
Outputs from Nature Communications
#9,881
of 57,717 outputs
Outputs of similar age
#12,008
of 339,952 outputs
Outputs of similar age from Nature Communications
#297
of 1,513 outputs
Altmetric has tracked 25,584,565 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 97th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 57,717 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 55.5. This one has done well, scoring higher than 82% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 339,952 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 96% of its contemporaries.
We're also able to compare this research output to 1,513 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 80% of its contemporaries.