| Title |
Structural basis for DNA bending by the architectural transcription factor LEF-1
|
|---|---|
| Published in |
Nature, August 1995
|
| DOI | 10.1038/376791a0 |
| Pubmed ID | |
| Authors |
John J. Love, Xiang Li, David A. Case, Klaus Giese, Rudolf Grosschedl, Peter E. Wright |
| Abstract |
Lymphoid enhancer-binding factor (LEF-1) and the closely related T-cell factor 1 (TCF-1) are sequence-specific and cell-type-specific DNA-binding proteins that play important regulatory roles in organogenesis and thymocyte differentiation. LEF-1 participates in regulation of the enhancer associated with the T cell receptor (TCR)-alpha gene by inducing a sharp bend in the DNA and facilitating interactions between Ets-1, PEBP2-alpha, and ATF/CREB, transcription factors bound at sites flanking the LEF-1 site. It seems that LEF-1 plays an architectural role in the assembly and function of this regulatory nucleoprotein complex. LEF-1 recognizes a specific nucleotide sequence through a high-mobility-group (HMG) domain. Proteins containing HMG domains bind DNA in the minor groove, bend the double helix, and recognize four-way junctions and other irregular DNA structures. Here we report the solution structure of a complex of the LEF-1 HMG domain and adjacent basic region with its cognate DNA. The structure reveals the HMG domain bound in the widened minor groove of a markedly distorted and bent double helix. The basic region binds across the narrowed major groove and contributes to DNA recognition. |
Mendeley readers
The data shown below were compiled from readership statistics for 126 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 2% |
| Portugal | 1 | <1% |
| Czechia | 1 | <1% |
| Austria | 1 | <1% |
| Spain | 1 | <1% |
| Canada | 1 | <1% |
| Unknown | 119 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 33 | 26% |
| Researcher | 26 | 21% |
| Professor > Associate Professor | 11 | 9% |
| Student > Master | 10 | 8% |
| Student > Bachelor | 8 | 6% |
| Other | 18 | 14% |
| Unknown | 20 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 42 | 33% |
| Biochemistry, Genetics and Molecular Biology | 36 | 29% |
| Chemistry | 13 | 10% |
| Computer Science | 4 | 3% |
| Medicine and Dentistry | 3 | 2% |
| Other | 8 | 6% |
| Unknown | 20 | 16% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 October 2014.
All research outputs
#7,203,348
of 22,768,097 outputs
Outputs from Nature
#64,834
of 90,828 outputs
Outputs of similar age
#6,996
of 23,737 outputs
Outputs of similar age from Nature
#110
of 199 outputs
Altmetric has tracked 22,768,097 research outputs across all sources so far. This one has received more attention than most of these and is in the 67th percentile.
So far Altmetric has tracked 90,828 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 99.3. This one is in the 28th percentile – i.e., 28% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 23,737 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.
We're also able to compare this research output to 199 others from the same source and published within six weeks on either side of this one. This one is in the 44th percentile – i.e., 44% of its contemporaries scored the same or lower than it.