Title |
Direct interaction of Plk4 with STIL ensures formation of a single procentriole per parental centriole
|
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Published in |
Nature Communications, October 2014
|
DOI | 10.1038/ncomms6267 |
Pubmed ID | |
Authors |
Midori Ohta, Tomoko Ashikawa, Yuka Nozaki, Hiroko Kozuka-Hata, Hidemasa Goto, Masaki Inagaki, Masaaki Oyama, Daiju Kitagawa |
Abstract |
Formation of one procentriole next to each pre-existing centriole is essential for centrosome duplication, robust bipolar spindle assembly and maintenance of genome integrity. However, the mechanisms maintaining strict control over centriole copy number are incompletely understood. Here we show that Plk4 and STIL, the key regulators of centriole formation, form a protein complex that provides a scaffold for recruiting HsSAS-6, a major component of the centriolar cartwheel, at the onset of procentriole formation. Furthermore, we demonstrate that phosphorylation of STIL by Plk4 facilitates the STIL/HsSAS-6 interaction and centriolar loading of HsSAS-6. We also provide evidence that negative feedback by centriolar STIL regulates bimodal centriolar distribution of Plk4 and seemingly restricts occurrence of procentriole formation to one site on each parental centriole. Overall, these findings suggest a mechanism whereby coordinated action of three critical factors ensures formation of a single procentriole per parental centriole. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Japan | 2 | 40% |
Unknown | 3 | 60% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 5 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 1 | <1% |
Germany | 1 | <1% |
Unknown | 195 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 54 | 27% |
Researcher | 36 | 18% |
Student > Master | 18 | 9% |
Student > Bachelor | 17 | 9% |
Student > Doctoral Student | 12 | 6% |
Other | 17 | 9% |
Unknown | 44 | 22% |
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Biochemistry, Genetics and Molecular Biology | 61 | 31% |
Neuroscience | 8 | 4% |
Medicine and Dentistry | 8 | 4% |
Chemistry | 5 | 3% |
Other | 3 | 2% |
Unknown | 48 | 24% |