| Title |
Rapid in vivo detection of isoniazid-sensitive Mycobacterium tuberculosis by breath test
|
|---|---|
| Published in |
Nature Communications, September 2014
|
| DOI | 10.1038/ncomms5989 |
| Pubmed ID | |
| Authors |
Seong W. Choi, Mamoudou Maiga, Mariama C. Maiga, Viorel Atudorei, Zachary D. Sharp, William R. Bishai, Graham S. Timmins |
| Abstract |
There is urgent need for rapid, point-of-care diagnostic tools for tuberculosis (TB) and drug sensitivity. Current methods based on in vitro growth take weeks, while DNA amplification can neither differentiate live from dead organisms nor determine phenotypic drug resistance. Here we show the development and evaluation of a rapid breath test for isoniazid (INH)-sensitive TB based on detection of labelled N2 gas formed specifically from labelled INH by mycobacterial KatG enzyme. In vitro data show that the assay is specific, dependent on mycobacterial abundance and discriminates between INH-sensitive and INH-resistant (S315T mutant KatG) TB. In vivo, the assay is rapid with maximal detection of (15)N2 in exhaled breath of infected rabbits within 5-10 min. No increase in (15)N2 is detected in uninfected animals, and the increases in (15)N2 are dependent on infection dose. This test may allow rapid detection of INH-sensitive TB. |
X Demographics
The data shown below were collected from the profiles of 51 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 18 | 35% |
| United Kingdom | 7 | 14% |
| Spain | 3 | 6% |
| India | 2 | 4% |
| Japan | 1 | 2% |
| France | 1 | 2% |
| Switzerland | 1 | 2% |
| Mexico | 1 | 2% |
| Italy | 1 | 2% |
| Other | 3 | 6% |
| Unknown | 13 | 25% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 30 | 59% |
| Scientists | 12 | 24% |
| Practitioners (doctors, other healthcare professionals) | 8 | 16% |
| Science communicators (journalists, bloggers, editors) | 1 | 2% |
Mendeley readers
The data shown below were compiled from readership statistics for 46 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 2% |
| France | 1 | 2% |
| Unknown | 44 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 11 | 24% |
| Student > Ph. D. Student | 7 | 15% |
| Student > Master | 6 | 13% |
| Student > Bachelor | 3 | 7% |
| Professor | 3 | 7% |
| Other | 7 | 15% |
| Unknown | 9 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 8 | 17% |
| Medicine and Dentistry | 7 | 15% |
| Biochemistry, Genetics and Molecular Biology | 6 | 13% |
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 7% |
| Chemistry | 3 | 7% |
| Other | 5 | 11% |
| Unknown | 14 | 30% |
Attention Score in Context
This research output has an Altmetric Attention Score of 78. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 December 2014.
All research outputs
#555,032
of 25,726,194 outputs
Outputs from Nature Communications
#9,504
of 58,230 outputs
Outputs of similar age
#5,471
of 263,785 outputs
Outputs of similar age from Nature Communications
#76
of 659 outputs
Altmetric has tracked 25,726,194 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 97th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 58,230 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 55.4. This one has done well, scoring higher than 83% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 263,785 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 97% of its contemporaries.
We're also able to compare this research output to 659 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 88% of its contemporaries.