| Title |
Long-term expansion of alveolar stem cells derived from human iPS cells in organoids
|
|---|---|
| Published in |
Nature Methods, October 2017
|
| DOI | 10.1038/nmeth.4448 |
| Pubmed ID | |
| Authors |
Yuki Yamamoto, Shimpei Gotoh, Yohei Korogi, Masahide Seki, Satoshi Konishi, Satoshi Ikeo, Naoyuki Sone, Tadao Nagasaki, Hisako Matsumoto, Shigeo Muro, Isao Ito, Toyohiro Hirai, Takashi Kohno, Yutaka Suzuki, Michiaki Mishima |
| Abstract |
The stable expansion of tissue-specific stem cells in vitro has contributed to research on several organs. Alveolar epithelial type II (AT2) cells function as tissue stem cells in the lung, but robust models for studying human AT2 cells are lacking. Here we report a method for the efficient generation and long-term expansion of alveolar organoids (AOs) harboring SFTPC(+) alveolar stem cells derived from human induced pluripotent stem cells (hiPSCs). hiPSC-derived SFTPC(+) cells self-renewed, with transcriptomes and morphology consistent with those of AT2 cells, and were able to differentiate into alveolar epithelial type I (AT1)-like cells. Single-cell RNA-seq of SFTPC(+) cells and their progenitors demonstrated that their differentiation process and cellular heterogeneity resembled those of developing AT2 cells in vivo. AOs were applicable to drug toxicology studies recapitulating AT2-cell-specific phenotypes. Our methods can help scientists overcome the limitations of current approaches to the modeling of human alveoli and should be useful for disease modeling and regenerative medicine. |
X Demographics
The data shown below were collected from the profiles of 50 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 6 | 12% |
| United States | 6 | 12% |
| United Kingdom | 2 | 4% |
| France | 1 | 2% |
| Unknown | 35 | 70% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 43 | 86% |
| Scientists | 4 | 8% |
| Science communicators (journalists, bloggers, editors) | 2 | 4% |
| Practitioners (doctors, other healthcare professionals) | 1 | 2% |
Mendeley readers
The data shown below were compiled from readership statistics for 261 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 261 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 49 | 19% |
| Student > Ph. D. Student | 47 | 18% |
| Student > Master | 29 | 11% |
| Student > Bachelor | 21 | 8% |
| Other | 14 | 5% |
| Other | 36 | 14% |
| Unknown | 65 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 62 | 24% |
| Agricultural and Biological Sciences | 32 | 12% |
| Medicine and Dentistry | 24 | 9% |
| Engineering | 15 | 6% |
| Pharmacology, Toxicology and Pharmaceutical Science | 12 | 5% |
| Other | 39 | 15% |
| Unknown | 77 | 30% |
Attention Score in Context
This research output has an Altmetric Attention Score of 52. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 March 2023.
All research outputs
#805,968
of 25,220,525 outputs
Outputs from Nature Methods
#1,056
of 5,344 outputs
Outputs of similar age
#16,755
of 329,026 outputs
Outputs of similar age from Nature Methods
#24
of 81 outputs
Altmetric has tracked 25,220,525 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 96th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
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We're also able to compare this research output to 81 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.