| Title |
Imaging RNA polymerase III transcription using a photostable RNA–fluorophore complex
|
|---|---|
| Published in |
Nature Chemical Biology, September 2017
|
| DOI | 10.1038/nchembio.2477 |
| Pubmed ID | |
| Authors |
Wenjiao Song, Grigory S Filonov, Hyaeyeong Kim, Markus Hirsch, Xing Li, Jared D Moon, Samie R Jaffrey |
| Abstract |
Quantitative measurement of transcription rates in live cells is important for revealing mechanisms of transcriptional regulation. This is particularly challenging when measuring the activity of RNA polymerase III (Pol III), which transcribes growth-promoting small RNAs. To address this issue, we developed Corn, a genetically encoded fluorescent RNA reporter suitable for quantifying RNA transcription in cells. Corn binds and induces fluorescence of 3,5-difluoro-4-hydroxybenzylidene-imidazolinone-2-oxime, which resembles the fluorophore found in red fluorescent protein (RFP). Notably, Corn shows high photostability, enabling quantitative fluorescence imaging of mTOR-dependent Pol III transcription. We found that, unlike actinomycin D, mTOR inhibitors resulted in heterogeneous transcription suppression in individual cells. Quantitative imaging of Corn-tagged Pol III transcript levels revealed distinct Pol III transcription 'trajectories' elicited by mTOR inhibition. Together, these studies provide an approach for quantitative measurement of Pol III transcription by direct imaging of Pol III transcripts containing a photostable RNA-fluorophore complex. |
X Demographics
The data shown below were collected from the profiles of 63 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 20 | 32% |
| United Kingdom | 5 | 8% |
| Japan | 4 | 6% |
| China | 2 | 3% |
| France | 2 | 3% |
| Canada | 1 | 2% |
| Denmark | 1 | 2% |
| Russia | 1 | 2% |
| Congo, The Democratic Republic of the | 1 | 2% |
| Other | 2 | 3% |
| Unknown | 24 | 38% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 39 | 62% |
| Scientists | 22 | 35% |
| Science communicators (journalists, bloggers, editors) | 2 | 3% |
Mendeley readers
The data shown below were compiled from readership statistics for 225 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 225 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 62 | 28% |
| Researcher | 31 | 14% |
| Student > Master | 21 | 9% |
| Student > Bachelor | 21 | 9% |
| Student > Doctoral Student | 17 | 8% |
| Other | 27 | 12% |
| Unknown | 46 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 76 | 34% |
| Chemistry | 49 | 22% |
| Agricultural and Biological Sciences | 27 | 12% |
| Neuroscience | 4 | 2% |
| Physics and Astronomy | 3 | 1% |
| Other | 16 | 7% |
| Unknown | 50 | 22% |
Attention Score in Context
This research output has an Altmetric Attention Score of 50. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 August 2023.
All research outputs
#860,476
of 25,753,578 outputs
Outputs from Nature Chemical Biology
#490
of 3,424 outputs
Outputs of similar age
#17,481
of 329,271 outputs
Outputs of similar age from Nature Chemical Biology
#8
of 52 outputs
Altmetric has tracked 25,753,578 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 96th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,424 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 26.7. This one has done well, scoring higher than 85% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 329,271 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 94% of its contemporaries.
We're also able to compare this research output to 52 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 84% of its contemporaries.