| Title |
Efficient CRISPR-Cas9–mediated genome editing in Plasmodium falciparum
|
|---|---|
| Published in |
Nature Methods, August 2014
|
| DOI | 10.1038/nmeth.3063 |
| Pubmed ID | |
| Authors |
Jeffrey C Wagner, Randall J Platt, Stephen J Goldfless, Feng Zhang, Jacquin C Niles |
| Abstract |
Malaria is a major cause of global morbidity and mortality, and new strategies for treating and preventing this disease are needed. Here we show that the Streptococcus pyogenes Cas9 DNA endonuclease and single guide RNAs (sgRNAs) produced using T7 RNA polymerase (T7 RNAP) efficiently edit the Plasmodium falciparum genome. Targeting the genes encoding native knob-associated histidine-rich protein (kahrp) and erythrocyte binding antigen 175 (eba-175), we achieved high (≥ 50-100%) gene disruption frequencies within the usual time frame for generating transgenic parasites. |
X Demographics
The data shown below were collected from the profiles of 12 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 3 | 25% |
| Brazil | 1 | 8% |
| United States | 1 | 8% |
| Denmark | 1 | 8% |
| Unknown | 6 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 7 | 58% |
| Scientists | 4 | 33% |
| Science communicators (journalists, bloggers, editors) | 1 | 8% |
Mendeley readers
The data shown below were compiled from readership statistics for 440 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Switzerland | 1 | <1% |
| France | 1 | <1% |
| Korea, Republic of | 1 | <1% |
| Australia | 1 | <1% |
| Brazil | 1 | <1% |
| Sweden | 1 | <1% |
| India | 1 | <1% |
| United Kingdom | 1 | <1% |
| Canada | 1 | <1% |
| Other | 5 | 1% |
| Unknown | 426 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 123 | 28% |
| Researcher | 87 | 20% |
| Student > Master | 52 | 12% |
| Student > Bachelor | 36 | 8% |
| Student > Doctoral Student | 22 | 5% |
| Other | 60 | 14% |
| Unknown | 60 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 172 | 39% |
| Biochemistry, Genetics and Molecular Biology | 125 | 28% |
| Immunology and Microbiology | 24 | 5% |
| Medicine and Dentistry | 21 | 5% |
| Chemistry | 9 | 2% |
| Other | 23 | 5% |
| Unknown | 66 | 15% |
Attention Score in Context
This research output has an Altmetric Attention Score of 41. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 June 2022.
All research outputs
#922,352
of 23,956,119 outputs
Outputs from Nature Methods
#1,201
of 5,088 outputs
Outputs of similar age
#9,434
of 233,640 outputs
Outputs of similar age from Nature Methods
#22
of 84 outputs
Altmetric has tracked 23,956,119 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 96th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 5,088 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 35.5. This one has done well, scoring higher than 76% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 233,640 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 95% of its contemporaries.
We're also able to compare this research output to 84 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 75% of its contemporaries.