| Title |
Redundant Functions for Nap1 and Chz1 in H2A.Z Deposition
|
|---|---|
| Published in |
Scientific Reports, September 2017
|
| DOI | 10.1038/s41598-017-11003-8 |
| Pubmed ID | |
| Authors |
Raghuvar Dronamraju, Srinivas Ramachandran, Deepak K. Jha, Alexander T. Adams, Julia V. DiFiore, Michael A. Parra, Nikolay V. Dokholyan, Brian D. Strahl |
| Abstract |
H2A.Z is a histone H2A variant that contributes to transcriptional regulation, DNA damage response and limits heterochromatin spreading. In Saccharomyces cerevisiae, H2A.Z is deposited by the SWR-C complex, which relies on several histone chaperones including Nap1 and Chz1 to deliver H2A.Z-H2B dimers to SWR-C. However, the mechanisms by which Nap1 and Chz1 cooperate to bind H2A.Z and their contribution to H2A.Z deposition in chromatin is not well understood. Using structural modeling and molecular dynamics simulations, we identify a series of H2A.Z residues that form a chaperone-specific binding surface. Mutation of these residues revealed different surface requirements for Nap1 and Chz1 interaction with H2A.Z. Consistent with this result, we found that loss of Nap1 or Chz1 individually resulted in mild defects in H2A.Z deposition, but that deletion of both Nap1 and Chz1 resulted in a significant reduction of H2A.Z deposition at promoters and led to heterochromatin spreading. Together, our findings reveal unique H2A.Z surface dependences for Nap1 and Chz1 and a redundant role for these chaperones in H2A.Z deposition. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 33% |
| Unknown | 2 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 67% |
| Scientists | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 26 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 31% |
| Researcher | 6 | 23% |
| Student > Bachelor | 2 | 8% |
| Student > Master | 2 | 8% |
| Professor | 1 | 4% |
| Other | 2 | 8% |
| Unknown | 5 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 12 | 46% |
| Biochemistry, Genetics and Molecular Biology | 7 | 27% |
| Psychology | 1 | 4% |
| Physics and Astronomy | 1 | 4% |
| Unknown | 5 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 May 2019.
All research outputs
#14,364,802
of 23,002,898 outputs
Outputs from Scientific Reports
#68,117
of 124,203 outputs
Outputs of similar age
#175,508
of 315,656 outputs
Outputs of similar age from Scientific Reports
#2,918
of 5,600 outputs
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