↓ Skip to main content

Virus and prokaryote enumeration from planktonic aquatic environments by epifluorescence microscopy with SYBR Green I

Overview of attention for article published in Nature Protocols, March 2007
Altmetric Badge

About this Attention Score

  • Good Attention Score compared to outputs of the same age (66th percentile)
  • Average Attention Score compared to outputs of the same age and source

Mentioned by

policy
1 policy source
twitter
2 X users

Citations

dimensions_citation
254 Dimensions

Readers on

mendeley
476 Mendeley
citeulike
3 CiteULike
Title
Virus and prokaryote enumeration from planktonic aquatic environments by epifluorescence microscopy with SYBR Green I
Published in
Nature Protocols, March 2007
DOI 10.1038/nprot.2007.6
Pubmed ID
Authors

Anand Patel, Rachel T Noble, Joshua A Steele, Michael S Schwalbach, Ian Hewson, Jed A Fuhrman

Abstract

Viruses are the most abundant biological entities in aquatic environments, typically exceeding the abundance of bacteria by an order of magnitude. The reliable enumeration of virus-like particles in marine microbiological investigations is a key measurement parameter. Although the size of typical marine viruses (20-200 nm) is too small to permit the resolution of details by light microscopy, such viruses can be visualized by epifluorescence microscopy if stained brightly. This can be achieved using the sensitive DNA dye SYBR Green I (Molecular Probes-Invitrogen). The method relies on simple vacuum filtration to capture viruses on a 0.02-microm aluminum oxide filter, and subsequent staining and mounting to prepare slides. Virus-like particles are brightly stained and easily observed for enumeration, and prokaryotic cells can easily be counted on the same slides. The protocol provides an inexpensive, rapid (30 min) and reliable technique for obtaining counts of viruses and prokaryotes simultaneously.

X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 476 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 9 2%
Canada 3 <1%
Germany 2 <1%
France 2 <1%
Turkey 1 <1%
Malaysia 1 <1%
Norway 1 <1%
Australia 1 <1%
Portugal 1 <1%
Other 4 <1%
Unknown 451 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 132 28%
Researcher 101 21%
Student > Master 39 8%
Student > Bachelor 32 7%
Student > Doctoral Student 22 5%
Other 70 15%
Unknown 80 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 182 38%
Environmental Science 70 15%
Biochemistry, Genetics and Molecular Biology 51 11%
Immunology and Microbiology 22 5%
Earth and Planetary Sciences 19 4%
Other 35 7%
Unknown 97 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 May 2014.
All research outputs
#7,202,427
of 24,983,099 outputs
Outputs from Nature Protocols
#1,985
of 2,899 outputs
Outputs of similar age
#27,924
of 86,832 outputs
Outputs of similar age from Nature Protocols
#62
of 98 outputs
Altmetric has tracked 24,983,099 research outputs across all sources so far. This one has received more attention than most of these and is in the 69th percentile.
So far Altmetric has tracked 2,899 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 16.4. This one is in the 30th percentile – i.e., 30% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 86,832 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.
We're also able to compare this research output to 98 others from the same source and published within six weeks on either side of this one. This one is in the 37th percentile – i.e., 37% of its contemporaries scored the same or lower than it.