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Reconstitution of mouse oogenesis in a dish from pluripotent stem cells

Overview of attention for article published in Nature Protocols, August 2017
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (55th percentile)

Mentioned by

4 tweeters
2 Facebook pages


31 Dimensions

Readers on

125 Mendeley
Reconstitution of mouse oogenesis in a dish from pluripotent stem cells
Published in
Nature Protocols, August 2017
DOI 10.1038/nprot.2017.070
Pubmed ID

Katsuhiko Hayashi, Orie Hikabe, Yayoi Obata, Yuji Hirao


This protocol is an extension to: Nat. Protoc. 8, 1513-1524 (2013); doi: 10.1038/nprot.2013.090; published online 11 July 2013Generation of functional oocytes in culture from pluripotent stem cells should provide a useful model system for improving our understanding of the basic mechanisms underlying oogenesis. In addition, it has potential applications as an alternative source of oocytes for reproduction. Using the most advanced mouse model in regard to reproductive engineering and stem cell biology, we previously developed a culture method that produces functional primorial germ cells starting from pluripotent cells in culture and described it in a previous protocol. This Protocol Extension describes an adaptation of this existing Protocol in which oogenesis also occurs in vitro, thus substantially modifying the technique. Oocytes generated from embryonic stem cells (ESCs) or induced pluripotent stem cells give rise to healthy pups. Here, we describe the protocol for oocyte generation in culture. The protocol is mainly composed of three different culture stages: in vitro differentiation (IVDi), in vitro growth (IVG), and in vitro maturation (IVM), which in total take ∼5 weeks. In each culture period, there are several checkpoints that enable the number of oocytes being produced in the culture to be monitored. The basic structure of the culture system should provide a useful tool for clarifying the complicated sequence of oogenesis in mammals.

Twitter Demographics

The data shown below were collected from the profiles of 4 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 125 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 125 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 21 17%
Student > Ph. D. Student 21 17%
Student > Bachelor 19 15%
Student > Master 13 10%
Student > Doctoral Student 8 6%
Other 19 15%
Unknown 24 19%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 40 32%
Agricultural and Biological Sciences 34 27%
Medicine and Dentistry 11 9%
Engineering 5 4%
Veterinary Science and Veterinary Medicine 3 2%
Other 7 6%
Unknown 25 20%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 August 2017.
All research outputs
of 11,623,235 outputs
Outputs from Nature Protocols
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Outputs of similar age
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Outputs of similar age from Nature Protocols
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Altmetric has tracked 11,623,235 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,797 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 9.7. This one is in the 20th percentile – i.e., 20% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 265,946 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 55% of its contemporaries.
We're also able to compare this research output to 44 others from the same source and published within six weeks on either side of this one. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.