Title |
Genome-wide DNA-methylation landscape defines specialization of regulatory T cells in tissues
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Published in |
Nature Immunology, August 2017
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DOI | 10.1038/ni.3799 |
Pubmed ID | |
Authors |
Michael Delacher, Charles D Imbusch, Dieter Weichenhan, Achim Breiling, Agnes Hotz-Wagenblatt, Ulrike Träger, Ann-Cathrin Hofer, Danny Kägebein, Qi Wang, Felix Frauhammer, Jan-Philipp Mallm, Katharina Bauer, Carl Herrmann, Philipp A Lang, Benedikt Brors, Christoph Plass, Markus Feuerer |
Abstract |
Regulatory T cells (Treg cells) perform two distinct functions: they maintain self-tolerance, and they support organ homeostasis by differentiating into specialized tissue Treg cells. We found that epigenetic modifications defined the molecular characteristics of tissue Treg cells. Tagmentation-based whole-genome bisulfite sequencing revealed more than 11,000 regions that were methylated differentially in pairwise comparisons of tissue Treg cell populations and lymphoid T cells. Similarities in the epigenetic landscape led to the identification of a common tissue Treg cell population that was present in many organs and was characterized by gain and loss of DNA methylation that included many gene sites associated with the TH2 subset of helper T cells, such as the gene encoding cytokine IL-33 receptor ST2, as well as the production of tissue-regenerative factors. Furthermore, the ST2-expressing population was dependent on the transcriptional regulator BATF and could be expanded by IL-33. Thus, tissue Treg cells integrate multiple waves of epigenetic reprogramming that define their tissue-restricted specialization. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 13 | 33% |
United Kingdom | 3 | 8% |
France | 2 | 5% |
Australia | 1 | 3% |
Malaysia | 1 | 3% |
Argentina | 1 | 3% |
Iraq | 1 | 3% |
El Salvador | 1 | 3% |
Turkey | 1 | 3% |
Other | 2 | 5% |
Unknown | 14 | 35% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 23 | 57% |
Scientists | 15 | 38% |
Science communicators (journalists, bloggers, editors) | 2 | 5% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 262 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 66 | 25% |
Researcher | 54 | 21% |
Student > Master | 27 | 10% |
Student > Doctoral Student | 15 | 6% |
Student > Bachelor | 12 | 5% |
Other | 32 | 12% |
Unknown | 56 | 21% |
Readers by discipline | Count | As % |
---|---|---|
Immunology and Microbiology | 76 | 29% |
Biochemistry, Genetics and Molecular Biology | 48 | 18% |
Agricultural and Biological Sciences | 38 | 15% |
Medicine and Dentistry | 20 | 8% |
Engineering | 4 | 2% |
Other | 15 | 6% |
Unknown | 61 | 23% |