| Title |
Pramel7 mediates ground-state pluripotency through proteasomal–epigenetic combined pathways
|
|---|---|
| Published in |
Nature Cell Biology, June 2017
|
| DOI | 10.1038/ncb3554 |
| Pubmed ID | |
| Authors |
Urs Graf, Elisa A. Casanova, Sarah Wyck, Damian Dalcher, Marco Gatti, Eva Vollenweider, Michal J. Okoniewski, Fabienne A. Weber, Sameera S. Patel, Marc W. Schmid, Jiwen Li, Jafar Sharif, Guido A. Wanner, Haruhiko Koseki, Jiemin Wong, Pawel Pelczar, Lorenza Penengo, Raffaella Santoro, Paolo Cinelli |
| Abstract |
Naive pluripotency is established in preimplantation epiblast. Embryonic stem cells (ESCs) represent the immortalization of naive pluripotency. 2i culture has optimized this state, leading to a gene signature and DNA hypomethylation closely comparable to preimplantation epiblast, the developmental ground state. Here we show that Pramel7 (PRAME-like 7), a protein highly expressed in the inner cell mass (ICM) but expressed at low levels in ESCs, targets for proteasomal degradation UHRF1, a key factor for DNA methylation maintenance. Increasing Pramel7 expression in serum-cultured ESCs promotes a preimplantation epiblast-like gene signature, reduces UHRF1 levels and causes global DNA hypomethylation. Pramel7 is required for blastocyst formation and its forced expression locks ESCs in pluripotency. Pramel7/UHRF1 expression is mutually exclusive in ICMs whereas Pramel7-knockout embryos express high levels of UHRF1. Our data reveal an as-yet-unappreciated dynamic nature of DNA methylation through proteasome pathways and offer insights that might help to improve ESC culture to reproduce in vitro the in vivo ground-state pluripotency. |
X Demographics
The data shown below were collected from the profiles of 12 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 8% |
| Turkey | 1 | 8% |
| Netherlands | 1 | 8% |
| Mexico | 1 | 8% |
| United Kingdom | 1 | 8% |
| Australia | 1 | 8% |
| United States | 1 | 8% |
| Unknown | 5 | 42% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 8 | 67% |
| Scientists | 4 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 75 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 75 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 16 | 21% |
| Researcher | 14 | 19% |
| Student > Master | 13 | 17% |
| Professor | 7 | 9% |
| Student > Postgraduate | 6 | 8% |
| Other | 10 | 13% |
| Unknown | 9 | 12% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 34 | 45% |
| Agricultural and Biological Sciences | 19 | 25% |
| Medicine and Dentistry | 5 | 7% |
| Immunology and Microbiology | 3 | 4% |
| Economics, Econometrics and Finance | 1 | 1% |
| Other | 1 | 1% |
| Unknown | 12 | 16% |
Attention Score in Context
This research output has an Altmetric Attention Score of 123. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 November 2023.
All research outputs
#326,617
of 24,778,793 outputs
Outputs from Nature Cell Biology
#136
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Outputs of similar age
#7,047
of 322,569 outputs
Outputs of similar age from Nature Cell Biology
#2
of 53 outputs
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