Peer 3
The majority of papers even in top journals do not do all (or even most of) the controls so it's a status quo. It will take too much time to check everything...
The majority of papers even in top journals do not do all (or even most of) the controls so it's a status quo. It will take too much time to check everything...
What are the y-axis scales (reads per million) used for the ChIP-seq tracks in Figures 2a and 3a? Are they equivalent? This information is really needed for interpretation and was omitted...
RE: "The names of these enzymes are confusing, and ACSS2 is the one that is either cytoplasmic or nuclear."Well, to me it is your comments that are confusing...
The DI in eGFP is about +8% (SD: ~22%) and this means there were two groups with + and - signs.Logically these two groups in eGFP (ideally) should be compared separately...
RE: Unregs @ June 3, 9:02pm and 10:16pm,I'm having trouble figuring out what each of you is asserting.Reading the bar graphs in Figure 4d, the means and SDs are roughly [5 ± 25] vs...
The names of these enzymes are confusing, and ACSS2 is the one that is either cytoplasmic or nuclear.
"SD error bars overlap"They are SDs. SEMs will be sqrt(n) or about 3 times smaller. The pooled SD of the difference will probably be about 30 and the SEM about 10...
Figure 4d‘ the spatial memory defect in ACSS2-knockdown mice manifests in a lowered discrimination index …compared to CONTROL MICE…Δ DI = − 29...
The paper is related to a new Mol Cell paper on the same enzyme.
The legend doesn't give the sample size and it should. However, the SEM values could be too small to resolve. Or n=1.The mean log2(fold-change) could be zero (or less - the figure doesn't show) and…
In figure 1f the SEM is missing for WT Q3 and Q4.Besides the KD Q1 and Q4 values are equal to 0 despite the presence of SEM.
I think that Fig 1F is fine.
What happened to SEM in Figure 1F ?
It will be interesting to test if the KO mice, with no overt phenotypes known yet (Sarah A. Comerford et al. Cell 2014), will show any memory deficits.
This is an interesting paper linking acetyl-CoA synthetase 2 to histone acetylation. According to the literature, this is a mitochrondria-associated protein, so the authors need to discuss if the…