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Antitumor activity of spinasterol isolated from Pueraria roots

Overview of attention for article published in Experimental and Molecular Medicine, April 2005
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (73rd percentile)
  • High Attention Score compared to outputs of the same age and source (90th percentile)

Mentioned by

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1 tweeter
patent
2 patents

Citations

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32 Dimensions

Readers on

mendeley
39 Mendeley
Title
Antitumor activity of spinasterol isolated from Pueraria roots
Published in
Experimental and Molecular Medicine, April 2005
DOI 10.1038/emm.2005.15
Pubmed ID
Authors

Gook-Che Jeon, Myoung-Soon Park, Do-Young Yoon, Chul-Ho Shin, Hong-Sig Sin, Soo-Jong Um

Abstract

We purified phytoestrogens from Pueraria root (Pueraria mirifica from Thailand and Pueraria lobata from Korea), which is used as a rejuvenating folk medicine in Thailand and China. Dried, powdered plant material was extracted with 100% ethanol and further separated by concentration, filtration, and thin layer silica gel chromatography. Using the fractions obtained during separation, we first investigated their cytotoxicity in several cancer cell lines from various tissues. The ethanol-extracted components (PE1, PE4) had significant antiproliferative effects on breast cancer cell lines, including MCF-7, ZR-75-1, MDA-MB-231, SK-BR-3, and Hs578T. Second, we compared these results with the cytotoxic effects of known flavonoids, sterols, and coumarins from Pueraria root. The known compounds were not as effective, and occurred in a different polarity region on HPLC. Third, further separation resulted in the isolation of eight different components (Sub PE-A to -H). One of these, PE-D, affected the growth of some breast cancer cell lines (MCF-7, MDA- MB-231) in a dose- and time-dependent manner, as well as the growth of ovarian (2774) and cervical cancer cells (HeLa). Finally, a transfection assay showed that this component had an estrogenic effect similar to 17beta - estradiol, which activates both estrogen receptor alpha (ERalpha) and ERbeta. The NMR analysis determined that spinasterol (stigmasta-7, 22-dien-3beta-ol) is an active cytotoxic component of Pueraria root.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 39 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
India 1 3%
Poland 1 3%
Denmark 1 3%
Italy 1 3%
Unknown 35 90%

Demographic breakdown

Readers by professional status Count As %
Student > Master 9 23%
Other 6 15%
Student > Ph. D. Student 6 15%
Student > Bachelor 4 10%
Professor 3 8%
Other 8 21%
Unknown 3 8%
Readers by discipline Count As %
Chemistry 8 21%
Agricultural and Biological Sciences 7 18%
Biochemistry, Genetics and Molecular Biology 6 15%
Medicine and Dentistry 5 13%
Pharmacology, Toxicology and Pharmaceutical Science 3 8%
Other 6 15%
Unknown 4 10%

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 December 2013.
All research outputs
#4,866,091
of 17,360,236 outputs
Outputs from Experimental and Molecular Medicine
#245
of 1,009 outputs
Outputs of similar age
#70,657
of 273,461 outputs
Outputs of similar age from Experimental and Molecular Medicine
#2
of 10 outputs
Altmetric has tracked 17,360,236 research outputs across all sources so far. This one has received more attention than most of these and is in the 70th percentile.
So far Altmetric has tracked 1,009 research outputs from this source. They receive a mean Attention Score of 4.7. This one has done well, scoring higher than 75% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 273,461 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.
We're also able to compare this research output to 10 others from the same source and published within six weeks on either side of this one. This one has scored higher than 8 of them.