| Title |
Directed differentiation of human induced pluripotent stem cells into functional cholangiocyte-like cells
|
|---|---|
| Published in |
Nature Protocols, March 2017
|
| DOI | 10.1038/nprot.2017.011 |
| Pubmed ID | |
| Authors |
Fotios Sampaziotis, Miguel Cardoso de Brito, Imbisaat Geti, Alessandro Bertero, Nicholas RF Hannan, Ludovic Vallier |
| Abstract |
The difficulty in isolating and propagating functional primary cholangiocytes is a major limitation in the study of biliary disorders and the testing of novel therapeutic agents. To overcome this problem, we have developed a platform for the differentiation of human pluripotent stem cells (hPSCs) into functional cholangiocyte-like cells (CLCs). We have previously reported that our 26-d protocol closely recapitulates key stages of biliary development, starting with the differentiation of hPSCs into endoderm and subsequently into foregut progenitor (FP) cells, followed by the generation of hepatoblasts (HBs), cholangiocyte progenitors (CPs) expressing early biliary markers and mature CLCs displaying cholangiocyte functionality. Compared with alternative protocols for biliary differentiation of hPSCs, our system does not require coculture with other cell types and relies on chemically defined conditions up to and including the generation of CPs. A complex extracellular matrix is used for the maturation of CLCs; therefore, experience in hPSC culture and 3D organoid systems may be necessary for optimal results. Finally, the capacity of our platform for generating large amounts of disease-specific functional cholangiocytes will have broad applications for cholangiopathies, in disease modeling and for screening of therapeutic compounds. |
X Demographics
The data shown below were collected from the profiles of 21 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 8 | 38% |
| Italy | 2 | 10% |
| United States | 1 | 5% |
| India | 1 | 5% |
| Finland | 1 | 5% |
| Canada | 1 | 5% |
| Belgium | 1 | 5% |
| Unknown | 6 | 29% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 10 | 48% |
| Members of the public | 10 | 48% |
| Science communicators (journalists, bloggers, editors) | 1 | 5% |
Mendeley readers
The data shown below were compiled from readership statistics for 144 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | <1% |
| Unknown | 143 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 29 | 20% |
| Student > Ph. D. Student | 27 | 19% |
| Student > Master | 19 | 13% |
| Student > Bachelor | 15 | 10% |
| Student > Doctoral Student | 8 | 6% |
| Other | 12 | 8% |
| Unknown | 34 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 40 | 28% |
| Agricultural and Biological Sciences | 24 | 17% |
| Medicine and Dentistry | 12 | 8% |
| Engineering | 11 | 8% |
| Pharmacology, Toxicology and Pharmaceutical Science | 5 | 3% |
| Other | 12 | 8% |
| Unknown | 40 | 28% |
Attention Score in Context
This research output has an Altmetric Attention Score of 26. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 December 2022.
All research outputs
#1,438,103
of 24,907,378 outputs
Outputs from Nature Protocols
#463
of 2,892 outputs
Outputs of similar age
#28,164
of 314,306 outputs
Outputs of similar age from Nature Protocols
#13
of 34 outputs
Altmetric has tracked 24,907,378 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 94th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,892 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 16.3. This one has done well, scoring higher than 84% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 314,306 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 91% of its contemporaries.
We're also able to compare this research output to 34 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 64% of its contemporaries.