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Genome-wide primary transcriptome analysis of H2-producing archaeon Thermococcus onnurineus NA1

Overview of attention for article published in Scientific Reports, February 2017
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Title
Genome-wide primary transcriptome analysis of H2-producing archaeon Thermococcus onnurineus NA1
Published in
Scientific Reports, February 2017
DOI 10.1038/srep43044
Pubmed ID
Authors

Suhyung Cho, Min-Sik Kim, Yujin Jeong, Bo-Rahm Lee, Jung-Hyun Lee, Sung Gyun Kang, Byung-Kwan Cho

Abstract

In spite of their pivotal roles in transcriptional and post-transcriptional processes, the regulatory elements of archaeal genomes are not yet fully understood. Here, we determine the primary transcriptome of the H2-producing archaeon Thermococcus onnurineus NA1. We identified 1,082 purine-rich transcription initiation sites along with well-conserved TATA box, A-rich B recognition element (BRE), and promoter proximal element (PPE) motif in promoter regions, a high pyrimidine nucleotide content (T/C) at the -1 position, and Shine-Dalgarno (SD) motifs (GGDGRD) in 5' untranslated regions (5' UTRs). Along with differential transcript levels, 117 leaderless genes and 86 non-coding RNAs (ncRNAs) were identified, representing diverse cellular functions and potential regulatory functions under the different growth conditions. Interestingly, we observed low GC content in ncRNAs for RNA-based regulation via unstructured forms or interaction with other cellular components. Further comparative analysis of T. onnurineus upstream regulatory sequences with those of closely related archaeal genomes demonstrated that transcription of orthologous genes are initiated by highly conserved promoter sequences, however their upstream sequences for transcriptional and translational regulation are largely diverse. These results provide the genetic information of T. onnurineus for its future application in metabolic engineering.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 29 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 6 21%
Researcher 4 14%
Student > Ph. D. Student 4 14%
Student > Bachelor 3 10%
Student > Doctoral Student 2 7%
Other 2 7%
Unknown 8 28%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 9 31%
Agricultural and Biological Sciences 5 17%
Immunology and Microbiology 2 7%
Earth and Planetary Sciences 1 3%
Chemistry 1 3%
Other 1 3%
Unknown 10 34%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 December 2018.
All research outputs
#18,534,624
of 22,955,959 outputs
Outputs from Scientific Reports
#93,786
of 123,945 outputs
Outputs of similar age
#237,630
of 310,302 outputs
Outputs of similar age from Scientific Reports
#3,337
of 4,593 outputs
Altmetric has tracked 22,955,959 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
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